# US 2015/0147409 A1 · Adipose stromal vascular fraction-conditioned medium

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- Number: US 2015/0147409 A1
- Document ID: US20150147409A1
- Status: Published application
- Kind: A1
- Application number: 14/550,400
- Filed: 2014-11-21
- Published: 2015-05-28
- Earliest claimed: 2013-11-22
- Assignees: Indiana University Research and Technology Corporation
- Applicants: Indiana University Research and Technology Corporation
- Inventors: Keith L. March; Brian H. Johnstone
- Web page: https://patentgrep.com/patent/US20150147409A1

## CPC

- A61K 35/35: Fat tissue; Adipocytes; Stromal cells; Connective tissues (adipose-derived stem cells A61K35/28; collagen A61K38/39)
- A61K 35/28: Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
- C12N 5/0653: {Adipocytes; Adipose tissue}
- C12N 5/0667: {Adipose-derived stem cells [ADSC]; Adipose stromal stem cells}
- C12N 2502/1305: Adipocytes
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A combination of therapeutic factors derived from non-adherent or poorly adherent stromal vascular fraction (SVF) cells exposed to protein-free basal medium are disclosed.

## Claims

### Claim 1

A conditioned medium obtained from digested adipose tissue.

### Claim 2 (depends on 1)

The conditioned medium of claim 1, wherein the digested adipose tissue is a fractioned adipose tissue.

### Claim 3 (depends on 2)

The conditioned medium of claim 2, wherein the fractioned adipose tissue is selected from the group consisting of adipose-derived stem cells and non-adipocyte, stromal-vascular fraction.

### Claim 4

A conditioned medium obtained from a non-adipocyte, stromal-vascular fraction.

### Claim 5 (depends on 4)

The conditioned medium of claim 4, wherein the conditioned medium is obtained from a suspension culture of the non-adipocyte, stromal-vascular fraction.

### Claim 6 (depends on 4)

The conditioned medium of claim 4, wherein the non-adipocyte, stromal-vascular fraction comprises at least one of a leukocyte and an endothelial cell.

### Claim 7

A method of producing conditioned medium, the method comprising: digesting adipose tissue by contacting the adipose tissue with an enzyme; fractionating the adipose tissue to obtain a fractionated adipose tissue that comprises at least a stromal-vascular fraction; culturing cells obtained from the fractionated tissue in a culture medium; and separating the cells from the culture medium to produce the conditioned medium.

### Claim 8 (depends on 7)

The method of claim 7, cells obtained from the stromal-vascular fraction are cultured in the culturing step.

### Claim 9 (depends on 8)

The method of claim 8, wherein the stromal-vascular fraction is selected from the group consisting of a freshly isolated population of stromal-vascular fraction and a non-adherent population of stromal-vascular fraction.

### Claim 10 (depends on 7)

The method of claim 7, further comprising culturing cells from digested adipose tissue after the digesting step for a sufficient time to allow a plurality of cells to adhere to a culture substrate; collecting non-adherent cells; culturing the non-adherent cells; and collecting culture medium after the culturing of the non-adherent cells, wherein the culture medium results in the conditioned medium.

### Claim 11 (depends on 10)

The method of claim 10, wherein the culture substrate comprises a plastic culture dish.

### Claim 12 (depends on 11)

The method of claim 11, wherein the plastic culture dish is an uncoated plastic culture dish.

### Claim 13 (depends on 10)

The method of claim 10, wherein the culturing the non-adherent cells comprises a suspension culture.

### Claim 14 (depends on 13)

The method of claim 13, wherein the conditioned medium is obtained from a non-adipocyte, stromal-vascular fraction of the adipose tissue.