Tissue culturing method, culturing method of ferns and explant obtained therefrom
AssigneeChunghwa Picture Tubes, Ltd.
InventorsChou-Chiang Kuo, Chin-Wen Ho, Kai-Ping Wang, Yu-Syuan Wu, Yung-Ting Tsai
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Abstract
A tissue culturing method includes following steps: providing a chopped gametophyte, generating calluses by culturing the chopped gametophyte, and performing apogamic regeneration of sporophytes, by culturing the calluses in a culture fluid to develop the sporophytes from the calluses. The present invention also provides a tissue culturing method of ferns and an explant.
Claims13
A culturing method of ferns, comprising:
- providing a chopped gametophyte of ferns;
- generating a callus, by culturing the chopped gametophyte of ferns till the chopped gametophyte generates the callus;
- and
- performing apogamic regeneration of a sporophyte, by culturing the calluses in a culture fluid to develop the sporophyte from the callus.
A tissue culturing method, comprising following steps:
- providing a chopped gametophyte;
- generating a callus, by culturing the chopped gametophyte till the chopped gametophyte generates the callus;
- and
- performing apogamic regeneration of a sporophyte, by culturing the calluses in a culture fluid to develop the sporophyte from the callus.
An explant, comprising a plurality of calluses massed in a round having a diameter between 0.19 cm and 0.25 cm.
Dependencies are inferred from the claim text.