Cyb5 and cyp17 mutations for alteration of 16-androstene steroid synthesis and reduced boar taint in pigs
AssigneesGenus, Inc., E.J. Squires, Ltd.
InventorE. James Squires
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Abstract
Novel mutations in cytochrome P450C17 (CYP17) and cytochrome b5 (CYB5) affecting 16-androstene steroid synthesis are disclosed. The novel mutations result in alterations in production of critical intermediaries in the synthesis of 16-androstene steroids. Altering the activity of these enzymes may be useful in enhancing reducing androstenone synthesis and reducing boar taint. The identification of these novel mutations also allows for the development of transgenic pigs bearing mutations in these enzymes or for genetic screening to identify pigs on the basis of their CYP17 and/or CYB5 genotype. Pigs having these mutations may be selected and bred to produce pigs that have a lower incidence of boar taint.
Claims19
An altered CYP17 protein that modifies 16-androstene steroid activity or production in pigs, said protein comprising:
- a modification of the amino acid present at one or more positions selected from the group consisting of:
- amino acids 102, 103, 104, 106, 108, 109, 112, 202, 344, 345, 348, 352 and 454 of the porcine CYP17 protein, compared to wild type (SEQ ID NO:4).
A method of modifying 16-androstene steroid activity to reducing boar taint in a pig comprising:
- introducing to said pig a polynucleotide that encodes a CYP17 protein, wherein amino acid 102, 103, 104, 106, 108, 112, 202, 344, 345, 348, 352 and 454 of the porcine CYP17 protein is modified from wild type (SEQ ID NO:4).
A genetically engineered pig having modified 16-androstene steroid activity said pig comprising:
- a modified CYP17 protein, wherein said modification includes a change at one or more positions selected from the group consisting of:
- amino acid 102, 103, 104, 106, 108, 112, 202, 344, 345, 348, 352 and 454 of the porcine CYP17 protein compared to wild type (SEQ ID NO:4).
An altered CYB5A protein that modifies 16-androstene steroid activity or production in pigs, said protein comprising:
- a modification of the amino acids at one or more positions selected from the group consisting of:
- amino acids 21, 28, 52, 57, 62 or 70 of said porcine CYB5A protein compared to wild type (SEQ ID NO:2).
A method of modifying 16-androstene steroid activity or production to reduce boar taint in a pig comprising;
- introducing to said pig a polynucleotide that encodes a CYB5A protein of said pig, wherein amino acids at one or more positions selected from the group consisting of:
- amino acids 21, 28, 52, 57, 62 or 70 of the porcine CYB5A protein is modified from wild type (SEQ ID NO:2).
A genetically engineered pig having modified 16-androstene steroid activity said pig comprising:
- a modified CYB5A protein, wherein said modification includes a change at amino acid 21, 28, 52, 57, 62 or 70 of the porcine CYB5A protein is modified from wild type (SEQ ID NO:2).
A method of identifying animals to determine those with altered 16-androstene steroid synthesis and concomitant boar taint characteristics, comprising:
- obtaining a sample of genetic material from said animal;
- and
- assaying for the presence of a genotype in said animal which is associated with improved boar taint, said genotype characterized by one or more of the following:
- a) a polymorphism in the CYP17 gene, said polymorphism resulting in a modification at one or more positions selected from the group consisting of:
- amino acid 102, 103, 104, 106, 108, 112, 202, 344, 345, 348, 352 and 454 of the porcine CYP17 protein;
- and
- b) a polymorphism in the CYB5 gene, said polymorphism resulting in a modification at one or more positions selected from the group consisting of:
- amino acid 21, 28, 52, 57, 62 or 70 of said porcine CYB5A protein.
An isolated polynucleotide comprising a nucleotide sequence encoding an altered CYB5A protein with modified 16-androstene steroid activity or production in pigs, said protein comprising:
- a modification of the amino acids at one or more positions selected from the group consisting of:
- amino acids 21, 28, 52, 57, 62 or 70 of said porcine CYB5A protein compared to wild type (SEQ ID NO:2).
An isolated polynucleotide comprising a nucleotide sequence encoding an altered CYP17 protein with modified 16-androstene steroid activity or production in pigs, said protein comprising:
- a modification of the amino acid present at one or more positions selected from the group consisting of:
- amino acids 102, 103, 104, 106, 108, 109, 112, 202, 344, 345, 348, 352 and 454 of the porcine CYP17 protein, compared to wild type (SEQ ID NO:4).
Dependencies are inferred from the claim text.