# US 2015/0104875 A1 · Algal elongase 6

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- Number: US 2015/0104875 A1
- Document ID: US20150104875A1
- Status: Published application
- Kind: A1
- Application number: 14/328,463
- Filed: 2014-07-10
- Published: 2015-04-16
- Earliest claimed: 2011-04-28
- Assignees: Aurora Algae, Inc.
- Applicants: Aurora Algae, Inc.
- Inventors: OLIVER KILIAN; Bertrand Vick
- Web page: https://patentgrep.com/patent/US20150104875A1

## CPC

- C12N 15/79: Vectors or expression systems specially adapted for eukaryotic hosts
- C12N 9/1029: {transferring groups other than amino-acyl groups (2.3.1)}
- C12N 9/93: {Ligases (6)}
- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12Y 602/01003: Long-chain-fatty-acid-CoA ligase (6.2.1.3)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

Provided herein are exemplary isolated nucleotide sequences encoding polypeptides having elongase activity, which utilize fatty acids as substrates.

## Claims

### Claim at position 1

1-16. (canceled)

### Claim 17

A vector comprising a polynucleotide sequence encoding elongase 6 having the amino acid sequence of SEQ ID NO:14.

### Claim 18 (depends on 17)

The vector of claim 17, wherein the polynucleotide sequence is SEQ ID NO:6.

### Claim 19 (depends on 17)

The vector of claim 17, further comprising a Violaxanthin-chlorophyll a binding protein (VCP) promoter.

### Claim 20 (depends on 17)

The vector of claim 17, which is a transformation vector.

### Claim 21 (depends on 17)

The vector of claim 17, which is a homologous recombination vector.

### Claim 22 (depends on 17)

The vector of claim 17, which has been incorporated into the genome of an algal cell.

### Claim 23

A method of modulating poly unsaturated fatty acid biosynthesis in an algal cell, the method comprising enhancing or suppressing the expression of elongase 6 having the amino acid sequence of SEQ ID NO:14.

### Claim 24 (depends on 23)

The method of claim 23, wherein the expression of elongase 6 is enhanced and EPA production is increased in the algal cell.

### Claim 25 (depends on 23)

The method of claim 23, wherein the expression of elongase 6 is suppressed and EPA production is decreased in the algal cell.

### Claim 26 (depends on 23)

The method of claim 23, wherein the algal cell is a Nannochloropsis cell.

### Claim 27 (depends on 24)

The method of claim 24, wherein the algal cell is present in a Nannochloropsis alga.

### Claim 28 (depends on 23)

The method of claim 23, wherein the algal cell has been transformed with a stronger or weaker promoter in front of a genomic sequence encoding elongase 6 having the amino acid sequence of SEQ ID NO:14.

### Claim 29 (depends on 25)

The method of claim 25, wherein the algal cell has been transformed to result in (1) an insertion in the genomic sequence encoding elongase 6 having the amino acid sequence of SEQ ID NO:14; or (2) a deletion or substitution of a portion or the full length of the genomic sequence encoding elongase 6.

### Claim 30

A transformed algal cell with altered poly unsaturated fatty acid biosynthesis, wherein the algal cell has altered expression level of elongase 6 having the amino acid sequence of SEQ ID NO:14.

### Claim 31 (depends on 30)

The algal cell of claim 30, wherein a stronger or weaker promoter is placed in front of a genomic sequence encoding elongase 6 having the amino acid sequence of SEQ ID NO:14.

### Claim 32 (depends on 30)

The algal cell of claim 30, which has (1) an insertion in the genomic sequence encoding elongase 6 having the amino acid sequence of SEQ ID NO:14; or (2) a deletion or substitution of a portion or the full length of the genomic sequence encoding elongase 6.

### Claim 33 (depends on 30)

The algal cell of claim 30, which is a Nannochloropsis cell.

### Claim 34 (depends on 33)

The algal cell of claim 33, which is a part of a Nannochloropsis alga.