Method of producing l-amino acids

A method of producing L-amino acids, the method comprising

• 1) culturing a recombinant coryneform microorganism capable of producing L-amino acids, wherein the recombinant coryneform microorganism is transformed by inserting an acetate-inducible promoter to the downstream of a stop codon of a target gene in a chromosome;
• and
• 2) adding acetate during the culturing to weaken the expression of the target gene, and to strengthen the L-amino acids production capability of the recombinant coryneform microorganism.