# US 2015/0104352 A1 · Use of disulfide bonds to form a reversible and reusable coating for nanofluidic devices

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- Number: US 2015/0104352 A1
- Document ID: US20150104352A1
- Status: Published application
- Kind: A1
- Application number: 14/053,956
- Filed: 2013-10-15
- Published: 2015-04-16
- Earliest claimed: 2013-10-15
- Assignees: International Business Machines Corporation
- Applicants: International Business Machines Corporation
- Inventors: Natalie Gunn; Jose M. Lobez Comeras; Priscilla Rogers; John Wagner
- Web page: https://patentgrep.com/patent/US20150104352A1

## CPC

- G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding}
- G01N 33/48721: {Investigating individual macromolecules, e.g. by translocation through nanopores (Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68)}
- G01N 33/54306: {Solid-phase reaction mechanisms}
- Y10T 436/143333: Saccharide [e.g., DNA, etc.]
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

A reusable coating for a nanopore structure is disclosed herein. A nanopore structure includes a substrate comprising a nanochannel and a monolayer of a chemical compound disposed onto at least a portion of a surface of the nanochannel. The chemical compound forms a reversible bond with at least one analyte binding compound introduced into the nanochannel. Methods for making and using the reusable coating are also disclosed.

## Claims

### Claim 1

A nanopore structure comprising:
a substrate comprising a nanochannel; and
a monolayer of a chemical compound disposed onto at least a portion of a surface of the nanochannel, wherein the chemical compound forms a reversible bond with at least one analyte binding compound introduced into the nanochannel.

### Claim 2 (depends on 1)

The nanopore structure of claim 1, wherein the at least one analyte binding compound comprises a functional group forming the reversible bond, and the reversible bond can be selectively severed.

### Claim 3 (depends on 1)

The nanopore structure of claim 1, wherein the analyte binding compound comprises an analyte binding group binding to an analyte.

### Claim 4 (depends on 3)

The nanopore structure of claim 3, wherein the analyte binding group is a protein, a peptide, an antibody, a small molecule, a polymer, a lipid, a polynucleotide, a DNA strand, a carbohydrate, or any combination thereof.

### Claim 5 (depends on 1)

The nanopore structure of claim 1, wherein the reversible bond is selectively severed by chemical methods, temperature methods, electrical methods, pH methods, or any combination thereof.

### Claim 6 (depends on 1)

The nanopore structure of claim 1, wherein the monolayer comprises a first sulfhydryl group.

### Claim 7 (depends on 6)

The nanopore structure of claim 6, wherein the analyte binding compound comprises a second sulfhydryl group and the reversible bond is a disulfide bond.

### Claim 8 (depends on 7)

The nanopore structure of claim 7, wherein the disulfide bond is severed with a reducing agent.

### Claim 9 (depends on 1)

The nanopore structure of claim 1, wherein the chemical compound of the monolayer comprises a functional group forming a contact product with the surface of the nanochannel and the functional group is an acrylate group, an acyl halide group, an amide group, an amine group, a carboxylate group, a carboxylate thiol group, an ester group, an ether group, a hydroxamic acid group, a hydroxyl group, a methacrylate group, a nitrate group, a nitrile group, a phosphate group, a phosphine group, a phosphonic acid group, a silane group, a sulfate group, a sulfide group, a sulfite group, a thiolate group, an alkene group, an alkyne group, an azide group, an acetal group, an aldehyde group, a diene group, an anhydride, or any combination thereof.

### Claim 10 (depends on 1)

The nanopore structure of claim 1, wherein the substrate further comprises a second nanochannel.

### Claim at position 11

11-20. (canceled)