Pest resistant plants
AssigneeKeygene N.V.
InventorsRobert Cornelis SCHUURINK, Michael Albertus Haring, Petronella Martina Bleeker
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Abstract
The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided.
Claims15
An isolated protein comprising the amino acid sequence of SEQ ID NO:
- 1 or an amino acid sequence comprising at least 99% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1 over the entire length.
A recombinant nucleic acid sequence comprising a heterologous promoter operably linked to a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence of SEQ ID NO:
- 2;
- (b) a nucleic acid sequence that encodes a polypeptide comprising an amino acid sequence of SEQ ID NO:
- 1;
- (c) a nucleic acid sequence that is at least 99% identical to the nucleic acid sequences of (a) or (b), and encodes a 7-epizingiberene synthase;
- (d) a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:1 wherein at least one amino acid is substituted, deleted, inserted or added and wherein the polypeptide is functionally equivalent to the polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 1 and
- (e) a nucleic acid sequence that hybridizes under stringent conditions to the nucleic acid sequences of (a), (b), or (c), and encodes a 7-epizingiberene synthase.
A cDNA sequence comprising a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence of SEQ ID NO:
- 2;
- (b) a nucleic acid sequence that is at least 99% identical to the nucleic acid sequence of (a) and encodes 7-epizingiberene synthase;
- and
- (c) a nucleic acid sequence that hybridizes under stringent conditions to the nucleic acid sequences of (a) and (b) and encodes a z-epizingiberene synthase.
A transgenic plant, plant cell, seed or fruit, comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1 or an amino acid sequence comprising at least 99% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1 over the entire length.
Dependencies are inferred from the claim text.