Dgat genes from yarrowia lipolytica for increased seed storage lipid production and altered fatty acid profiles in soybean
AssigneeE. I. Du Pont de Nemours and Company
InventorsHOWARD GLENN DAMUDE, WILLIAM D. HITZ, KNUT MEYER, NARENDRA S. YADAV
Life of this patent
Estimating the term…
Abstract
Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from Yarrowia Lipolytica are used to achieve the increase in seed storage lipids.
Claims14
A transgenic soybean seed having increased total fatty acid content of at least 10% when compared to the total fatty acid content of a non-transgenic, null segregant soybean seed.
2-48. (canceled)
A transgenic soybean seed having an increased total fatty acid content of at least 10% when compared to the total fatty acid content of a non-transgenic, null segregant soybean seed, wherein the percentage of fatty acids in said soybean seed that are represented by linolenic acid is decreased, and wherein said seed comprises a recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence, and wherein said at least one DGAT sequence comprises the nucleic acid sequence encoding a polypeptide having at least 95% sequence idnetoty to the polypeptide having the amino acid sequence set forth in SEQ ID NO:65.
A method for increasing the total fatty acid content of a soybean comprising:
- a) transforming at least one soybean cell with a recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence, wherein said at least one DGAT sequence comprises the nucleic acid sequence encoding a polypeptide having at least 95% sequence identity to the polypeptide having the amino acid sequence set forth in SEQ ID NO:65;
- and
- b) selecting the transformed soybean cell(s) of step(a) having an increased total fatty acid content of a non-transgenic, null segregant soybean seed, wherein the percentage of fatty acids in said soybean seed that are represented by linolenic acid is decreased.
Meal obtained from a transgenic soybean seed having an increased total fatty acid content of at least 10% when compared to the total fatty acid content of a recombinant construct having at least one diacylglycerol acyltransferase (DGAT) sequence, and wherein said at least one DGAT sequence comprises the nucleotide sequence encoding a polypeptide having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:10, 65, 83, 88 or 93, and further wherein the soluble carbohydrate content in said meal is decreased compared to the soluble carbohydrate content of a meal obtained from a non-transgenic, null segregant seed.
A method for decreasing the soluble carbohydrate content of a meal obtained from a soybean seed, the method comprising the steps of:
- a) transforming at least one regenerable soybean cell with at least one recombinant construct comprising a nucleotide sequence encoding a polypeptide having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:10, 65, 83, 88 or 93;
- b) regenerating a transgenic plant comprising the recombinant DNA construct from the regenerable soybean cell of a);
- and
- c) selecting the transgenic plant of step b), or a transgenic progeny plant from the plant of step b), wherein transgenic seed of the transgenic plant or the transgenic progeny plant comprises the recombinant DNA construct, and wherein expression of said recombinant construct in the transgenic seed results in the transgenic seed having a decreased soluble carbohydrate content compared to the soluble carbohydrate content of a meal obtained from a non-transgenic, null segregant seed.
Dependencies are inferred from the claim text.