Inducible promoter sequences for regulated expression and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsAndrew Cigan, Erica Unger-Wallace
Life of this patent
Estimating the term…
Abstract
The plant promoter of a CBSU-Anther_Subtraction library (CAS1) gene encoding a mannitol dehydrogenase, and fragments thereof, and their use in promoting the expression of one or more heterologous nucleic acid fragments in an inducible manner in plants are described. These promoter fragments are also useful in creating recombinant DNA constructs comprising nucleic acid sequences encoding a desired gene product operably linked to such promoter fragments which can be utilized to transform plants and bring the expression of the gene product under external chemical and/or heat control in monocotyledonous and dicotyledonous plants.
Claims22
A recombinant DNA construct comprising:
- a) a nucleotide sequence comprising any one of the sequences set forth in SEQ ID NO:9 or SEQ ID NO:10, or a full-length complement thereof;
- b) a nucleotide sequence comprising a functional fragment of SEQ ID NO:10, or a full-length complement thereof;
- c) a nucleotide sequence comprising a sequence having at least 85% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a) or (b);
- d) a nucleotide sequence which hybridizes to SEQ ID NO:9 under highly stringent conditions of a wash of 0.1 SSC, 0.1% (w/v) SDS at 65° C.;
- e) a nucleotide sequence comprising all or a fragment of a 1.7 kb 5′ non-coding sequence of a mannitol dehydrogenase;
- or,
- f) a derivative of one of the nucleotide sequences indicated in (a), (b), (c), (d) or (e) obtained by substitution, addition and/or deletion of one or more nucleotides;
- and,
- wherein said nucleotide sequence is an inducible promoter.
3-4. (canceled)
(canceled)
9-11. (canceled)
(canceled)
17-20. (canceled)
A plant stably transformed with a recombinant expression construct comprising a plant promoter and a heterologous nucleic acid fragment operably linked to said promoter, wherein said promoter is an inducible promoter and capable of controlling expression of said heterologous nucleic acid fragment in a plant cell, and further wherein said promoter comprises a fragment of SEQ ID NO:10.
24-29. (canceled)
(canceled)
A recombinant DNA construct comprising:
- a) a nucleotide sequence comprising all or a functional fragment of SEQ ID NO:
- 19 or SEQ ID NO:
- 22;
- b) a nucleotide sequence comprising a full-length complement of the nucleotide sequence (a);
- or,
- c) a nucleotide sequence comprising a sequence having at least 90% sequence identity, based on the BLASTN method of alignment, when compared to the nucleotide sequence of (a) or (b);
- and,
- wherein said nucleotide sequence is a promoter.
Dependencies are inferred from the claim text.