Methods and compositions for peronospora resistance in spinach
AssigneeSeminis Vegetable Seeds, Inc.
InventorsBart Willem Brugmans, Eduardo López Fernandez, Inge Weber
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Abstract
The present disclosure provides for unique spinach plants with broad-spectrum resistance to downy mildew and their progeny. Such plants may comprise an introgressed QTL associated with the broad-spectrum resistance to downy mildew. In certain aspects, compositions, including distinct polymorphic molecular markers, and methods for producing, using, identifying, selecting, and the like of plants or germplasm with resistance to downy mildew are provided.
Claims42
A Spinacia oleracea spinach plant comprising in its genome an introgressed locus from Spinacia tetrandra that confers broad-spectrum resistance to Peronospora farinosa f. sp. Spinaciae.
A recombined chromosomal segment comprising an allele from Spinacia tetrandra conferring broad-spectrum resistance to Peronospora farinosa f. sp. spinaciae and lacking all or some genetic loci that are genetically linked thereto in Spinacia tetrandra.
A method of selecting a plant with broad-spectrum resistance to Peronospora farinosa f. sp. spinaciae comprising selecting said plant based on the presence in the genome of the plant of at least a first genetic polymorphism in or in genetic linkage disequilibrium with a chromosomal segment from Spinacia tetrandra conferring said resistance, wherein the locus is flanked in the Spinacia tetrandra genome by loci corresponding to SEQ ID NOs:1 or 2, or sequences at least 95% identical thereto.
A method for producing an agronomically elite spinach plant that comprises in its genome at least one locus conferring broad-spectrum resistance to Peronospora farinosa f. sp. spinaciae (Pfs), the method comprising:
- (i) crossing a first spinach plant with a second spinach plant, wherein the first plant comprises in its genome at least one locus from Spinacia tetrandra that confers broad-spectrum resistance to Peronospora farinosa f. sp. Spinaciae, and wherein the second plant lacks said locus;
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- (ii) selecting at least a first progeny spinach plant resulting from said crossing that comprises said locus.
A method of producing an agronomically elite spinach plant comprising broad-spectrum resistance to Peronospora farinosa f. sp. spinaciae (Pfs), comprising introgressing into an agronomically elite Spinacia oleracea plant a chromosomal segment from Spinacia tetrandra that confers the resistance, wherein said locus is defined as located in a region of the Spinacia tetrandra genome flanked by loci corresponding to the Spinacia tetrandra allele in SEQ ID NOs:1 or 2, or sequences at least 95% identical thereto.
A spinach plant or progeny thereof obtainable by introgressing a locus from S. tetrandra conferring broad-spectrum resistance to Peronospora farinosa f. sp. spinaciae (Pfs) into S. oleracea.
Dependencies are inferred from the claim text.