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US 2015/0101070 A1Published application
Method for screening patient-specific anti-cancer agent using limiting dilution assay
AssigneeSamsung Life Public Welfare Foundation
InventorsDo Hyun Nam, Kyeung Min Joo, YeonSook Choi
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Abstract
A screening method is described for selecting patient-specific anti-cancer agents reflecting individual genetic properties, in a precise and rapid manner, using an extremely small amount of cancer cells. Such screening method is useful for development of novel anti-cancer agents and the personalized medical field.
Claims28
A method for screening patient-specific anti-cancer agents comprising:
- (a) three-dimensional culturing cancer cells isolated from patient-derived cancer tissues into 0.01 to 0.1 μl of hydrogel;
- (b) treating the three-dimensional cultured cancer cells with candidate anti-cancer agents;
- and (c) confirming whether or not sphere of the cancer cell is formed, and selecting the candidate anti-cancer agent as a patient-specific anti-cancer agent, which is confirmed to show a sphere-forming inhibitory activity.
A method for screening patient-specific anti-cancer agents comprising:
- (a) three-dimensional culturing cancer cells isolated from patient-derived cancer tissue into 0.01 to 0.1 μl of hydrogel;
- (b) treating the three-dimensional cultured cancer cell with a candidate anti-cancer agent;
- (c) confirming whether or not sphere of the cancer cell is formed, and selecting the candidate anti-cancer agent as a patient-specific anti-cancer agent, which is confirmed to show a sphere-forming inhibitory activity;
- and (d) treating an animal model containing patient-derived cancer stem cells with the patient-specific anti-cancer agent selected in the step (c) and confirming an anti-cancer effect.
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Dependencies are inferred from the claim text.