Anuran cross-species molecular sensors
AssigneeArizona Board of Regents On Behalf of Northern Arizona University
InventorsCatherine R. Propper, Stephen M. Beckstrom-Sternberg, Caren C. Helbing, Nicholas J. Veldhoen
Life of this patent
Estimating the term…
Abstract
Described herein are DNA primer sequences designed for the determination of gene or transcript information from Anuran species, and which may be used in studies for developmental and/or toxicity testing and for environmental toxicology or ecological assessment. Also described herein is a rapid, sensitive, high-throughput assay useful for supporting potential risk assessment across vertebrate clades, and that is also useful for evaluation of complex contaminant mixtures.
Claims9
A frog tadpole metamorphosis assay method comprising a first phase and a second phase, wherein:
- the first phase comprises exposing a tadpole to a compound of interest, removing first and second portions of the tadpole's tail, subjecting the first portion to an apatosis assay, and
- the second phase comprises subjecting the second portion to an assay to detect shifts in a quantity of pre-determined mRNA transcripts.
A composition of matter comprising a primer pair selected from the group consisting of primer pairs A, B, C, D, E, F, G, H, I, J, K, L, M, N, and O:
- Primer
- First
- SEQ ID
- Second
- SEQ ID
- Pair
- Primer
- NO:
- Primer
- NO:
- A
- CTGGTGGTGGATAT
- 1
- GAGTCTTAAGTCA
- 2
- GATGGT
- CGGCAAG
- B
- TACAGCTTCACCAC
- 3
- TCCACATCTGCTG
- 4
- CACAG
- GAAGGT
- C
- TTTGCYTGGCGKCA
- 5
- ARCRGCACTGCGY
- 6
- CTTTT
- CTGTA
- D
- TGGCTGARGGATTY
- 7
- ACAGCAGAGCAGG
- 8
- TAYACC
- CAAMGA
- E
- TGATAAGGCCACAG
- 9
- CGGGTGATCTTGT
- 10
- GRTACCACTA
- CGATRA
- F
- CTCATAGAAGAAAA
- 11
- GAAGGCTTCTAAG
- 12
- CAGAGAAAARAGA
- TCCACTTTTCC
- G
- CCTGGCTCTSTAYG
- 13
- RGCTGATCCRAAR
- 14
- ACTC
- TTGAC
- H
- ASCTCCRCAGAAYC
- 15
- TCACGTACCAGGC
- 16
- AGCA
- CAAAA
- I
- CYGCTCAGTGTCTG
- 17
- ARGGGCCGGTACT
- 18
- GTGT
- TGTTT
- J
- GTTGCCATGARGGW
- 19
- CCATTGCCWGTGC
- 20
- GGAC
- AGATA
- K
- TACAACAGCTGCAC
- 21
- TCCTTGTCATAGT
- 22
- TGAT
- CTTCTGT
- L
- CAGGGGACAGAGAA
- 23
- TGAGCTTTCTTGC
- 24
- AAGGTG
- CACAG
- M
- GCTGCTGGTGTTGG
- 25
- AGCATGTTGTCAC
- 26
- TGART
- CRTTCC
- N
- GCTWCAYCCAGAGT
- 27
- GCTGYTCCTCCTG
- 28
- GGTCGTT
- CTCCA
- O
- YATGACYGATGCTG
- 29
- CATAWCCCTTTGG
- 30
- TTCTAG
- TGTTGC
A normalizer gene panel comprising two or more primer pairs selected from the group consisting of primer pairs A, B, C, D, L, M, and N:
- Primer
- First
- SEQ ID
- Second
- SEQ ID
- Pair
- Primer
- NO:
- Primer
- NO:
- A
- CTGGTGGTGGATAT
- 1
- GAGTCTTAAGTCA
- 2
- GATGGT
- CGGCAAG
- B
- TACAGCTTCACCAC
- 3
- TCCACATCTGCTG
- 4
- CACAG
- GAAGGT
- C
- TTTGCYTGGCGKCA
- 5
- ARCRGCACTGCGY
- 6
- CTTTT
- CTGTA
- D
- TGGCTGARGGATTY
- 7
- ACAGCAGAGCAGG
- 8
- TAYACC
- CAAMGA
- L
- CAGGGGACAGAGAA
- 23
- TGAGCTTTCTTGC
- 24
- AAGGTG
- CACAG
- M
- GCTGCTGGTGTTGG
- 25
- AGCATGTTGTCAC
- 26
- TGART
- CRTTCC
- N
- GCTWCAYCCAGAGT
- 27
- GCTGYTCCTCCTG
- 28
- GGTCGTT
- CTCCA
A thyroid hormone responsive gene panel comprising two or more primer pairs selected from the group consisting of primer pairs A, B, D, E, F, G, H, I, J, K, and O:
- Primer
- First
- SEQ ID
- Second
- SEQ ID
- Pair
- Primer
- NO:
- Primer
- NO:
- A
- CTGGTGGTGGATAT
- 1
- GAGTCTTAAGTCA
- 2
- GATGGT
- CGGCAAG
- B
- TACAGCTTCACCAC
- 3
- TCCACATCTGCTG
- 4
- CACAG
- GAAGGT
- D
- TGGCTGARGGATTY
- 7
- ACAGCAGAGCAGG
- 8
- TAYACC
- CAAMGA
- E
- TGATAAGGCCACAG
- 9
- CGGGTGATCTTGT
- 10
- GRTACCACTA
- CGATRA
- F
- CTCATAGAAGAAAA
- 11
- GAAGGCTTCTAAG
- 12
- CAGAGAAAARAGA
- TCCACTTTTCC
- G
- CCTGGCTCTSTAYG
- 13
- RGCTGATCCRAAR
- 14
- ACTC
- TTGAC
- H
- ASCTCCRCAGAAYC
- 15
- TCACGTACCAGGC
- 16
- AGCA
- CAAAA
- I
- CYGCTCAGTGTCTG
- 17
- ARGGGCCGGTACT
- 18
- GTGT
- TGTTT
- J
- GTTGCCATGARGGW
- 19
- CCATTGCCWGTGC
- 20
- GGAC
- AGATA
- K
- TACAACAGCTGCAC
- 21
- TCCTTGTCATAGT
- 22
- TGAT
- CTTCTGT
- O
- YATGACYGATGCTG
- 29
- CATAWCCCTTTGG
- 30
- TTCTAG
- TGTTGC
Dependencies are inferred from the claim text.