Sodium hypochlorite resistant genes
AssigneeSapphire Energy, Inc.
InventorsChristopher Yohn, Eric N. Hampton
Life of this patent
Estimating the term…
Abstract
The present disclosure provides novel genes, identified from a Chlamydomonas reinhardtii cDNA library that when over expressed in algae confer sodium hypochlorite resistance to the organism. The present disclosure also provides methods of using the novel genes, and organism transformed by the novel genes.
Claims39
1-111. (canceled)
A modified non-vascular photosynthetic comprising an organism transformed with a polynucleotide sequence comprising:
- a nucleic acid sequence encoding an amino acid sequence comprising one of SEQ ID NO:
- 43, SEQ ID NO:
- 45, SEQ ID NO:
- 47, SEQ ID NO:
- 49, SEQ ID NO:
- 53, SEQ ID NO:
- 55, SEQ ID NO:
- 57, SEQ ID NO:
- 59, SEQ ID NO:
- 61, SEQ ID NO:
- 65, SEQ ID NO:
- 67, SEQ ID NO:
- 69, SEQ ID NO:
- 71, SEQ ID NO:
- 73, SEQ ID NO:
- 75, or SEQ ID NO:
- 77;
- wherein said organism is tolerant to sodium hypochlorite at a concentration of from 0.01 ppm to 1 ppm, from 1 ppm to 5 ppm, from 5 ppm to 10 ppm, from 10 ppm to 15 ppm, from 15 ppm to 20 ppm, from 20 ppm to 25 ppm, from 25 ppm to 30 ppm, from 30 ppm to 35 ppm, from 35 ppm to 40 ppm, from 40 ppm to 45 ppm, from 45 ppm to 50 ppm, from 50 ppm to 55 ppm, from 55 ppm to 60 ppm, from 60 ppm to 65 ppm, from 65 ppm to 70 ppm, from 70 ppm to 75 ppm, from 75 ppm to 80 ppm, from 80 ppm to 85 ppm, from 85 ppm to 90 ppm, from 90 ppm to 95 ppm, from 95 ppm to 100 ppm, from 100 ppm to 200 ppm, from 200 ppm to 500 ppm, from 500 ppm to 1000 ppm, from 1000 ppm to 2000 ppm, from 2000 ppm to 5000 ppm, from 5000 ppm to 10000 ppm, or from 10000 ppm to 15000 ppm as compared to an unmodified organism of the same species.
A modified non-vascular photosynthetic comprising an organism transformed with a polynucleotide sequence comprising a nucleic acid sequence encoding an amino acid sequence comprising one of SEQ ID NO:
- 43, SEQ ID NO:
- 45, SEQ ID NO:
- 47, SEQ ID NO:
- 49, SEQ ID NO:
- 53, SEQ ID NO:
- 55, SEQ ID NO:
- 57, SEQ ID NO:
- 59, SEQ ID NO:
- 61, SEQ ID NO:
- 65, SEQ ID NO:
- 67, SEQ ID NO:
- 69, SEQ ID NO:
- 71, SEQ ID NO:
- 73, SEQ ID NO:
- 75, or SEQ ID NO:
- 77;
- wherein said modified organism has an increased growth rate as compared to an untransformed organism.
A modified higher plant comprising a higher plant transformed with a polynucleotide sequence comprising a nucleic acid sequence encoding an amino acid sequence comprising one of SEQ ID NO:
- 43, SEQ ID NO:
- 45, SEQ ID NO:
- 47, SEQ ID NO:
- 53, SEQ ID NO:
- 55, SEQ ID NO:
- 57, SEQ ID NO:
- 59, SEQ ID NO:
- 61, SEQ ID NO:
- 65, SEQ ID NO:
- 67, SEQ ID NO:
- 69, SEQ ID NO:
- 71, SEQ ID NO:
- 73, SEQ ID NO:
- 75, or SEQ ID NO:
- 77:
- wherein said modified organism has an increased growth rate as compared to an untransformed higher plant.
Dependencies are inferred from the claim text.