Compositions and methods of gene silencing in plants
AssigneesUniversity of Georgia Research Foundation, Inc., The Board of Trustees of the University of Illinois
InventorsThomas B. Jacobs, Wayne A. Parrott, Lila O. Vodkin
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Abstract
Compositions and methods for inducing gene silencing events in plants are disclosed. The compositions typical include a polynucleotide encoding an miRNA target sequence operably linked to a sequence of from a target gene, cDNA or mRNA, or fragment thereof. When expressed in the presence of an miRNA specific for the miRNA target sequence the compositions can induce production of trans-acting siRNA that silence the target of interest. Transgenic plants and preferred plant pathways that can be targeted using the disclosed methods and compositions are also disclosed.
Claims20
A recombinant nucleic acid construct comprising a tasiRNA-inducing miRNA recognition sequence selected from the group consisting of SEQ ID NOs 5, 1, 2, 3, 4, or 6 and a coding region of a target gene.
A method of reducing gene expression in a plant cell comprising transforming the plant cell with a vector that expresses recombinant nucleic acid construct comprising a tasiRNA-inducing miRNA recognition sequence selected from the group consisting of SEQ ID NOs 5, 1, 2, 3, 4, or 6 and a coding region of a target gene,
- wherein co-expression of an miRNA that binds to the miRNA target sequence induces production of trans-acting siRNA in the cell.
A method of reducing gene expression in a plant comprising transforming a plant cell with a vector that expresses a recombinant nucleic acid construct comprising a tasiRNA-inducing miRNA recognition sequence selected from the group consisting of SEQ ID NOs 5, 1, 2, 3, 4, or 6 and a coding region of a target gene,
- wherein co-expression of an miRNA that binds to the miRNA target sequence induces production of trans-acting siRNA in the cell.
A transgenic plant transformed with a recombinant nucleic acid construct comprising a tasiRNA-inducing miRNA recognition sequence selected from the group consisting of SEQ ID NOs 5, 1, 2, 3, 4, or 6 and a coding region of a target gene.
Dependencies are inferred from the claim text.