Plant Germplasm Resistant to RNA Viruses
AssigneeKansas State University Research Foundation
InventorsJohn Fellers, Harold N. Trick, Luisa Cruz, Jessica Rupp
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Abstract
Disclosed is a dsRNA construct used to silencing specific eukaryotic translation initiation factor in plants to produce a plant resistant to viruses such as Potyviruses, Luteoviruses, and Furoviruses. More specifically, the plant would be resistant to viruses such as Wheat streak mosaic virus, Triticum mosaic virus, Soil bourne mosaic virus, or Barley yellow dwarf virus. Also disclosed are non-transgenic wheat plants having the genes for eIF(iso)4E-2 or eIF4G silenced.
Claims34
A method of producing a plant germplasm having resistance to RNA viruses, the method comprising:
- a) introducing into a parental plant germplasm a chimeric DNA molecule comprising (i) a plant expressible promoter, (ii) a region which encodes dsRNA for eIF(iso)4E-2 or eIF4G which is capable of inhibiting RNA viral replication (iii) plant translation termination signal,
- b) transforming said parental plant germplasm,
- c) generating a plant germplasm from the parental plant germplasm comprising the chimeric DNA molecule;
- and
- d) selecting plant germplasm obtained from step (c) and identifying germplasm having immunity to viral RNA replication.
A transgenic plant having resistance to RNA viruses, the transgenic plant comprising a chimeric DNA molecule which encodes a double stranded RNA molecule for eIF(iso)4E-2 or eIF4G, wherein the transgenic plant is resistant to RNA viruses.
A transgenic plant comprising a nucleotide sequence that encodes a sequence that encodes an eIF(iso)4E-2 polynucleotide sequence of SEQ. ID. NO:
- 1 or an eIF(iso)4E-2 polynucleotide sequence comprising an antisense sequence corresponding to SEQ. ID. NO:
- 1, or encodes an eIF4G polynucleotide sequence of SEQ. ID. NO:
- 3 or an eIF4G polynucleotide sequence comprising an antisense sequence corresponding to SEQ. ID. NO:
- 3, wherein said transgenic plant has increased resistance to positive strand RNA viruses.
A nucleic acid construct comprising a nucleotide sequence of SEQ ID NO:1 or an antisense sequence corresponding to SEQ ID NO:1, operably linked to a promoter that drives expression in a plant cell.
A nucleic acid construct comprising a nucleotide sequence of SEQ. ID. NO:
- 3 or an antisense sequence corresponding to SEQ. ID. NO:3, operably linked to a promoter that drives expression in a plant cell.
A non-transgenic plant comprising an eIF(iso)4E-2 allele in its genome, whereby the eIF(iso)4E-2 allele is an allele which encodes a protein comprising the amino acid sequence of SEQ. ID. NO:
- 2, characterized in that said eIF(iso)4E-2 allele comprises one or more mutations in its nucleotide sequence and whereby as a result of said one or more mutations the plant comprising said mutant allele in its genome has significant resistance to RNA viruses compared to a plant comprising a wild type eIF(iso)4E-2 allele in its genome.
A non-transgenic plant comprising an eIF4G allele in its genome, whereby the eIF4G allele is an allele which encodes a protein comprising the amino acid sequence of SEQ. ID. NO:
- 4, characterized in that said eIF4G allele comprises one or more mutations in its nucleotide sequence and whereby as a result of said one or more mutations the plant comprising said mutant allele in its genome has significant resistance to RNA viruses compared to a plant comprising a wild type eIF4G allele in its genome.
Dependencies are inferred from the claim text.