US 2015/0089683 A1Published application

Transgenic Plants With Enhanced Traits

AssigneeMonsanto Technology LLC

InventorsMark Scott Abad, Pranesh Badami, Amarjit Basra, Erin Bell, Christopher P. Bonin, Paolo Castiglioni, Eric R. Cerny, Jaishree M. Chittoor-Vijayanath, Paul Chomet, Todd Dezwaan, Stephen Duff, Michael D. Edgerton, Barry S. Goldman, Balasulojini Karunanandaa, Kempagangaiah Krishnamurthy, Ping Li, Adrian A. Lund, Llinda Lutfiyya, Thomas M. Malvar, Donald E. Nelson, Monnanda S. Rajani, Dhanalakshmi Ramachandra, Thomas G. Ruff, Sara Salvador, S. Sangeetha, Char Shobha, Jeffrey M. Staub, Virginia Ursin, Tyamagondlu V. Venkatesh, Kammaradi R. Vidya, Xiaoyun Wu, Sean Yang, Jianmin Zhao

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Abstract

This disclosure provides transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

Claims11

Dependent claims

A plant comprising a recombinant DNA molecule comprising a polynucleotide encoding a polypeptide, wherein the nucleotide sequence of the polynucleotide is selected from the group consisting of:

a) a nucleotide sequence set forth as SEQ ID NO:
1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 157, or 159;
b) a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:
2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96-153, 158, 160, or 162-169;
c) a nucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 157, or 159;
d) a nucleotide sequence encoding a protein with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96-153, 158, 160, or 162-169;
and
e) a nucleotide sequence encoding a protein with at least 70% identity to one of SEQ ID NO:
76 and 84, wherein said nucleotide sequence encodes a Glucose-6-phosphate 1-dehydrogenase;
wherein said plant has an enhanced trait as compared to a control plant.

9 more elements

A method for producing a plant comprising:

introducing into a plant cell a recombinant DNA molecule comprising a polynucleotide encoding a polypeptide, wherein the nucleotide sequence of the polynucleotide is selected from the group consisting of:
a) a nucleotide sequence set forth as SEQ ID NO:
1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 157, or 159;
b) a nucleotide sequence encoding a protein having the amino acid sequence of SEQ ID NO:
2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96-153, 158, 160, or 162-169;
c) a nucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 157, or 159;
d) a nucleotide sequence encoding a protein with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to SEQ ID NO:
2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96-153, 158, 160, or 162-169;
and
e) a nucleotide sequence encoding a protein with at least 70% identity to one of SEQ ID NO:
76 and 84, wherein said nucleotide sequence encodes a Glucose-6-phosphate 1-dehydrogenase;
and growing a plant from said plant cell.

10 more elements

Dependencies are inferred from the claim text.

Facts

Application: 14/395,636Filed 6 Mar 2013
Priority: 20 Apr 2012
Published: 26 Mar 2015
Term ends: Not estimatedThe source records don’t support an estimate.

Classification16 codes

C12N 15/8261
Phenotypically and genetically modified plants via recombinant DNA technology: with agronomic traits, e.g. crop yield
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C07K 14/81
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: protease inhibitors
C12N 5/04
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor: plant cells…