Polymer micelle complex including nucleic acid
AssigneeThe University of Tokyo
InventorsKazunori KATAOKA, Yuichi YAMASAKI, Nobuhiro NISHIYAMA, Woo-Dong JANG, Anwar ARNIDA
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Abstract
It is an object of the present invention to provide: a polyion complex that sufficiently retains a photosensitizing substance in serum and is excellent in terms of structural stability; a nucleic acid polyplex as a constituent thereof; and a device and a kit for delivering a nucleic acid into a cell. The nucleic acid polyplex of the present invention comprises a cationic polymer represented by general formula (1) and a nucleic acid. The polyion complex of the present invention comprises the nucleic acid polyplex of the present invention and an anionic photosensitizing substance.
Claims19
A nucleic acid polyplex, which comprises a cationic polymer represented by the following general formula (1) and a nucleic acid:
- wherein each of R1 and R2 independently represents a hydrogen atom or a substitutable linear or branched alkyl group containing 1 to 12 carbon atoms;
- each of R3 and R4 independently represents a residue derived from an amine compound having a primary amine;
- R5 represents a residue containing a thiol group or a substituent thereof;
- L1 represents NH, CO, or a group represented by the following general formula (5) or (6):
- —(CH2)p1—NH— (5)
- -L2a-(CH2)q1-L3a- (6)
- wherein p1 represents an integer between 1 and 5, L2a represents OCO, OCONH, NHCO, NHCOO, NHCONH, CONH or COO, L3a represents NH or CO, and q1 represents an integer between 1 and 5;
- a represents an integer between 100 and 500;
- b represents an integer between 5 and 100;
- c represents an integer between 20 and 100;
- the symbol “/” indicates that the ratio of the numbers and the sequence order of monomer units described at the left and right sides thereof are arbitrarily determined.
A kit for delivering a nucleic acid into a cell, which comprises a cationic polymer represented by the following general formula (1) and an anionic photosensitizing substance:
- wherein each of R1 and R2 independently represents a hydrogen atom or a substitutable linear or branched alkyl group containing 1 to 12 carbon atoms;
- each of R3 and R4 independently represents a residue derived from an amine compound having a primary amine;
- R5 represents a residue containing a thiol group or a substituent thereof;
- L1 represents NH, CO, or a group represented by the following general formula (5) or (6):
- —(CH2)p1—NH— (5)
- -L2a-(CH2)q1-L3a- (6)
- wherein p1 represents an integer between 1 and 5, L2a represents OCO, OCONH, NHCO, NHCOO, NHCONH, CONH or COO, L3a represents NH or CO, and q represents an integer between 1 and 5:
- a represents an integer between 100 and 500;
- b represents an integer between 5 and 100;
- c represents an integer between 20 and 100;
- and
- the symbol “/” indicates that the ratio of the numbers and the sequence order of monomer units described at the left and right sides thereof are arbitrarily determined.
A cationic polymer represented by the following general formula (1):
- wherein each of R1 and R2 independently represents a hydrogen atom or a substitutable linear or branched alkyl group containing 1 to 12 carbon atoms;
- each of R3 and R4 independently represents a residue derived from an amine compound having a primary amine;
- R5 represents a residue containing a thiol group or a substituent thereof;
- L1 represents NH, CO, or a group represented by the following general formula (5) or (6):
- —(CH2)p1—NH— (5)
- -L2a-(CH2)q1-L3a- (6)
- wherein p1 represents an integer between 1 and 5, L2a represents OCO, OCONH, NHCO, NHCOO, NHCONH, CONH or COO, L3a represents Nil or CO, and q1 represents an integer between 1 and 5;
- a represents an integer between 100 and 500;
- b represents an integer between 5 and 100;
- c represents an integer between 20 and 100;
- and
- the symbol “/” indicates that the ratio of the numbers and the sequence order of monomer units described at the left and right sides thereof are arbitrarily determined.
Dependencies are inferred from the claim text.