US 2015/0087584 A1 · METHOD FOR EFFECTIVE MANAGEMENT OF HELICOVERPA ARMIGERA

Abstract

The present invention relates to a method for pest management using inhibitory repeat domain IRD 9 (Seq ID No.2) proteinase inhibitor showing enhanced inhibitory activity against the gut proteases of insects. More particularly, the present invention relates to a IRD 9 (Seq ID No.2) proteinase inhibitor from non-host plant Capsicum annuum, which possesses significantly high insect protease inhibition activity against the gut proteases of Helicoverpa armigera.

Claims3

Dependent claims

A method for effective management of Helicoverpa armigera using inhibitory repeat domain IRD-9, a Pin-II type proteinase inhibitor (PIs), the method comprising:

a. providing IRD-9 having Seq Id no. 2 from non-host plant Capsicum annuum,
b. cloning of IRD-9 in Pichia pastoris (Yeast expression system) in pPIC-9 vector,
c. expressing and purifying IRD-9 protein,
d. feeding IRD-9 protein to Helicoverpa armigera in artificial diet, and
e. calculating growth parameters for antibiosis effect of IRD-9.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/370,278Filed 7 Jan 2013

Priority

IN 0035/DEL/20125 Jan 2012

Published

26 Mar 2015

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

A01N 43/50
Having rings with two nitrogen atoms as the only ring hetero atoms: 1,3-Diazoles; Hydrogenated 1,3-diazoles
A01N 37/46
Containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton…
A01N 65/38
Magnoliopsida [dicotyledons]: solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants

Method for effective management of helicoverpa armigera

Life of this patent

Method for effective management of helicoverpa armigera

Life of this patent

Abstract

Claims3