Early trimester screening for early- and late-onset preeclampsia

A method for predicting a pregnant woman's risk for developing early-onset preeclampsia, the method comprising:

• measuring concentrations of one or more metabolites selected from the group consisting of acetate, alanine, arginine, choline, creatine, dimethylamine, acetamide, trimethylamine, glutamine, citrate, ethanol, formate, glycerol, glycine, leucine, methanol, methionine, ornithine, phenylalanine, propylene glycol, serine, succinate, hydroxy-isovalerate, pyruvate, hydroxy-buturate and threonine in the pregnant woman's bodily fluid;
• measuring crown rump length (CRL) of the pregnant woman's fetus;
• comparing the pregnant woman's one or more metabolite concentrations to concentrations of corresponding one or more metabolites obtained from pregnant women with early-onset preeclampsia and to concentrations of the corresponding one or more metabolites obtained from pregnant women exhibiting normal blood pressure and normal protein levels in urine, wherein all metabolite concentrations are standardized according to CRL average values from pregnant women with early-onset preeclampsia and pregnant women exhibiting normal blood pressure and normal protein levels in urine;
• and
• c) predicting the pregnant woman's risk of developing early-onset preeclampsia, wherein a statistically significant change in the concentration of the one or more metabolites between the pregnant woman and the corresponding one or more standardized metabolites from the pregnant women exhibiting normal blood pressure and normal protein levels in urine indicates a greater probability of developing early-onset preeclampsia.

A method for predicting a pregnant woman's risk for developing late-onset preeclampsia, the method comprising:

• a) measuring concentrations of one or more metabolites selected from the group consisting of 2-hydroxy-butyrate, acetate, carnitine, creatine, glucose, glycerol, alanine, valine, pyruvate and methylhistidine in the pregnant woman's bodily fluid;
• b) comparing the pregnant woman's one or more metabolite concentrations to the corresponding one or more metabolite concentrations obtained from pregnant women with late-onset preeclampsia and pregnant women exhibiting normal blood pressure and normal protein levels in urine, wherein all metabolite concentrations are measured at the same or similar gestational age;
• and
• c) predicting the pregnant woman's risk of developing late-onset preeclampsia, wherein the statistically significant change in concentration of one or more metabolites between the pregnant woman and the corresponding one or more metabolites from the pregnant women exhibiting normal blood pressure and normal protein levels in urine indicates a greater probability of developing late-onset preeclampsia.