Methods for screening voltage-gated proteins
AssigneesD.E. Shaw Research, LLC, D.E. Shaw Research India Technology Private Limited
InventorsMorten Ostergaard JENSEN, David BORHANI, VISHWANATH Jogini
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Abstract
In one aspect, the invention relates to a method for identifying a compound which modulates the activity of a voltage-gated protein. In certain embodiments, the voltage gate protein is a voltage-gated ion channel. In certain embodiments, the voltage-gated protein is a voltage sensitive phosphatase. In certain embodiments, the voltage-gated protein used in conjunction with the methods of the invention is modified to altered permeability or voltage sensitivity.
Claims35
A method for identifying a compound which modulates the activity of a voltage-gated ion channel, the method comprising
- (a) providing a voltage-gated ion channel in a structure that separates a first medium from a second medium, wherein the voltage-gated ion channel exhibits independent ion permeation,
- (b) contacting the voltage-gated ion channel with a test compound,
- (c) measuring the amount of said independent ion permeation through the voltage-gated ion channel between the first and second media, and
- (d) comparing the amount of said independent ion permeation measured for the voltage-gated ion channel contacted with the test compound to the amount of said independent ion permeation measured for the voltage-gated ion channel not contacted with the test compound, wherein an increase or decrease in the amount of said independent ion permeation of the voltage-gated ion channel contacted with the test compound compared to the voltage-gated ion channel not contacted with the test compound indicates that the test compound modulates the activity of the voltage-gated ion channel.
A method for identifying a compound which modulates the activity of a voltage-sensitive phosphatase, the method comprising
- (a) providing a voltage-sensitive phosphatase in a structure that separates a first medium from a second medium,
- (b) contacting the voltage-sensitive phosphatase with a test compound,
- (c) measuring the activity of the voltage-sensitive phosphatase, and
- (d) comparing the amount of said activity measured for the voltage-sensitive phosphatase contacted with the test compound to the amount of said activity measured for the voltage-sensitive phosphatase not contacted with the test compound, wherein an increase or decrease in the amount of activity of the voltage-sensitive phosphatase contacted with the test compound compared to the voltage-sensitive phosphatase not contacted with the test compound indicates that the test compound modulates the activity of the voltage-sensitive phosphatase.
Dependencies are inferred from the claim text.