Device, Array, And Methods For Disease Detection And Analysis
AssigneeLawrence Livermore National Security, LLC
InventorsRupa S. Rao, Stephen M. Lane, Dennis L. Matthews, Matthew A. Coleman
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Abstract
A device and array coupled to capture molecules are provided. Specifically, the device and array can be used for detecting the presence and concentration of biomarkers in a sample from a subject. The device and array can also allow the use of a method for scoring a sample for, e.g., the purpose of diagnosing a disease. The method can also be advantageous to applications where there is a need to accurately determine the disease stage of a subject for the purpose of making therapeutic decisions.
Claims5
A multiplex immunoassay detection device for measurement of breast cancer markers in a patient sample, comprising:
- a glass solid support comprising a plurality of distinct spots arranged in an array format, each spot comprising a distinct capture antibody capable of binding to a breast cancer marker, the distinct capture antibody selected from the group consisting of antibody Clone 191924 for HER2, antibody Clone 36006.211 for MMP-2, antibody Clone M8071022 for CA-15-3 and antibody Clone 190312 for osteopontin;
- a glass cover plate, wherein the glass cover plate forms an upper surface positioned above the solid support;
- a vertical support comprising adhesive silicone, wherein the vertical support forms a connection between the solid support and the cover plate, the connection forming at least one channel surrounding the array of capture antibodies, and wherein the channel comprises a first end and a second end and wherein the first end of the channel comprises an opening;
- and
- an absorbent material comprising a nitrocellulose membrane connected to the second end.
Dependencies are inferred from the claim text.