Screening method for the detection of clostridium difficile
AssigneeUniversity College Cardiff Consultants Limited
InventorsLesley William James Baillie, Lovleen Tina Joshi
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Abstract
The invention concerns a screening method for the detection of Clostridium difficile in a sample, wherein said method comprises the detection of conserved target regions in the Clostridium genome through the binding of at least one oligonucleotide probe to said target site.
Claims34
A diagnostic method for the detection of C. difficile in a sample wherein said C. difficile comprises at least one nucleic acid target region selected from one of the following sequences:
- I)
- (SEQ ID NO:
- 27)
- Atggatttgaatactttgcacctgctaatacggatgctaacaacatag
- aa;
- II)
- (SEQ ID NO:
- 2)
- Aaaatattactttaatactaacactgctgttgcagttactggatggcaa
- actattaatggtaaaaatactacttt;
- III)
- (SEQ ID NO:
- 28)
- Ttggcaaataagctatcttttaactttagtgataaacaagatgtacctg
- ta;
- IV)
- (SEQ ID NO:
- 29)
- Catattctggtatattaaatttcaataataaaatttactat;
- and
- V)
- (SEQ ID NO:
- 30)
- Tttgagggagaatcaataaactatactggttggttagatttagatgaaa
- aga;
- which method comprises exposing said sample to:
- a) at least one oligonucleotide probe that is complementary to at least a part of at least one of said target regions, or;
- b) at least one oligonucleotide probe that is complementary to at least a part of at least one degenerate version of said target regions or;
- c) an oligonucleotide probe that is at least 80% homologous to the oligonucleotides in a) or b);
- and
- detecting the binding of said oligonucleotide to said target region and, where binding occurs, concluding that C. difficile is present in said sample.
(canceled)
A kit for the detection of C. difficile in a sample wherein said C. difficile comprises at least one nucleic acid target region selected from one of the following sequences:
- I)
- (SEQ ID NO:
- 27)
- Atggatttgaatactttgcacctgctaatacggatgctaacaacataga
- a;
- II)
- (SEQ ID NO:
- 2)
- Aaaatattactttaatactaacactgctgttgcagttactggatggcaa
- actattaatggtaaaaatactacttt;
- III)
- (SEQ ID NO:
- 28)
- Ttggcaaataagctatcttttaactttagtgataaacaagatgtacctg
- ta;
- IV)
- (SEQ ID NO:
- 29)
- Catattctggtatattaaatttcaataataaaatttactat;
- and
- V)
- (SEQ ID NO:
- 30)
- Tttgagggagaatcaataaactatactggttggttagatttagatgaaa
- aga;
- which kit comprises:
- a) at least one oligonucleotide probe that is complementary to at least a part of at least one of said target regions, or;
- b) at least one oligonucleotide probe that is complementary to at least a part of at least one degenerate version of said target regions or;
- c) an oligonucleotide probe that is at least 80% homologous to the oligonucleotides in a) or b);
- and
- optionally, reagents and/or instructions for practising said detection.
(canceled)
Dependencies are inferred from the claim text.