Affinity reagents for protein purification
AssigneesImpossible Foods Inc., The Board of Trustees of the Leland Stanford Junior University
InventorsPatrick Brown, R. Edward Watts, Pehr Harbury
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Abstract
Disclosed herein are methods and compositions for purifying proteins from crude solutions.
Claims96
A composition comprising a non-polypeptide pH switchable reagent which binds a target at a first pH and does not bind the target at a second pH.
A consumable product comprising a pH switchable reagent.
A composition comprising a protein content, wherein the protein content is obtained from a plant;
- wherein the composition is substantially free of odorants found in the plant.
A composition comprising a protein content, wherein the protein content is obtained from a plant;
- wherein the composition is substantially free of colorants found in the plant.
A method for identifying a non-polypeptide pH switchable reagent, the method comprising:
- a. generating a library of non-polypeptides by tag-directed synthesis;
- b. exposing at least a subset of the library to a protein in a solution at a first pH;
- c. altering the pH of the solution to a second pH;
- and
- d. identifying a non-polypeptide pH switchable peptoid reagent which binds a target at a first pH and does not bind the target at a second pH.
A method for isolating one or more target proteins comprising:
- a. applying a composition comprising the one or more target proteins to a substrate;
- wherein the substrate comprises one or more non-polypeptide pH switchable reagents;
- wherein at least part of the applying step occurs at a first pH;
- wherein at the first pH at least one of the one or more target proteins binds to the one or more non-polypeptide pH switchable reagents;
- b. releasing the target proteins by adjusting the pH of the solution to a second pH;
- wherein at the second pH at least one or more of the target proteins does not bind the non-polypeptide pH switchable reagent and;
- c. collecting the target proteins that eluted from the one or more non-polypeptide pH switchable reagents.
Dependencies are inferred from the claim text.