Device for bacteria classification and pretreatment device for bacteria test

A method for bacteria classification using a bacterial classification device, the method comprising the steps of:

• taking an image in a petri dish including bacterial colonies therein by an imaging unit;
• displaying the image in the petri dish by a display unit;
• classifying the bacterial colonies in the petri dish based on color data including brightness and saturation, and a shape and an outline including an area and degree of a perfect circle with respect to each bacterial colony by a classification unit;
• outputting an image which has an operator set the number of the classified bacterial colonies in advance, and then inputting the specified number of the classified bacterial colonies by a data input/output unit;
• and
• reclassifying the bacterial colonies once classified by the classification unit based on the number of the classified bacterial colonies specified by the operator and the color data including brightness and saturation, and the shape and the outline including the area and degree of the perfect circle with respect to each bacterial colony by a bacterial colonies classification processing unit, wherein
• an instruction for increasing/decreasing the number of candidate bacterial colonies is inputted by the data input/output unit, corresponding to the classification results of the bacterial colonies in the petri dish, in accordance with the number of the classified bacterial colonies specified by the operator;
• the bacterial colonies classification processing unit classifies the bacterial colonies following a specified degree of the increased/decreased number of the bacterial colonies inputted by the data input/output unit;
• and
• the bacterial colonies classification processing unit represents the color data including brightness and saturation, and the shape and the outline including the area and degree of the perfect circle with respect to each bacterial colony by a Mahalanobis' generalized distance, thereby to perform the classification by modulating a threshold following the specified degree of the increased/decreased number of the bacterial colonies.