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AssigneeIndustry-Academic Cooperation Foundation, Yonsei University
InventorsJung Ho HWANG, Yun Haeng JOE
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A method for quantitatively evaluating the antimicrobial activity of an antimicrobial sample using a disc diffusion method, and a measurement system using the method are provided. The method comprises: (a) measuring a maximum inhibition zone width (IZWmax) for a first sample, in which a colony is expected not to be observed, using a disc diffusion method, and measuring a colony forming unit (CFU0) value for the first sample using a colony counting method; (b) measuring a microbial growth inhibition zone width (IZWi) and a colony forming unit (CFUi) value for a second sample having any antimicrobial activity; (c) calculating α value and β value based on the results of steps (a) and (b) using equations (10) and (20); (d) measuring a microbial growth inhibition zone width (IZWx) for a measurement sample; and (e) calculating a quantitative antimicrobial activity value (η) using equation (30).
A method for quantitatively evaluating an antimicrobial activity of an antimicrobial sample, calculating a quantitative antimicrobial activity value (η) for a measurement sample by measuring a microbial growth inhibition zone width (IZWx) for a measurement sample using a maximum inhibition zone width (IZWmax), a colony forming unit (CFU0) value for a first sample, in which a colony is expected not to be observed, a microbial growth inhibition zone width (IZWi), a colony forming unit (CFUi) for a second sample containing a concentration ρareal of an antimicrobial agent and a ρareal.
A system for measuring an antimicrobial activity of a sample, the system comprising:
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