US 2015/0087008 A1 · Method For Monitoring A Reaction And To Reaction System For Implementing Same

Abstract

A method for monitoring a reaction and a reaction system are provided. The reaction system includes at least one vessel for the reaction medium, which is in fluid communication with an injection tube; at least one vessel for a carrier fluid that is immiscible with the reaction medium, which is in fluid communication with a reaction tube; the injection tube being mounted so as to lead into the reaction tube such that individual drops of the reaction medium can be injected into the reaction tube and into the immiscible carrier fluid, so as to form a train of reaction chambers; at least one detector for monitoring a reaction; a means for classifying the reaction chambers; and at least one means for recirculating reaction chambers in front of at least one detector for monitoring a reaction.

Claims23

Dependent claims

A reaction system, notably of cultures of microorganisms, comprising:

at least one reservoir of reaction mixture fluidically connected to a capillary injection tube;
at least one reservoir of a carrier fluid that is immiscible with the reaction mixture, fluidically connected to a capillary reaction tube,
the capillary injection tube being mounted opening into the capillary reaction tube so that individual drops of reaction mixture are injectable into the capillary reaction tube, into the immiscible carrier fluid, so as to form a succession of reactors,
at least one reaction monitoring detector,
wherein the system comprises a means of referencing the reactors for identifying them uniquely in the succession of reactors, and at least one means for recirculating the reactors in front of at least one reaction monitoring detector.

2 more elements

A method of monitoring a reaction in a reaction mixture, wherein in the method comprises the following steps:

a) filling a capillary reaction tube with a carrier medium that is immiscible with the reaction mixture;
b) injecting, by means of a capillary injection tube, an individual drop of reaction mixture in the capillary reaction tube, into the immiscible carrier fluid;
c) circulating the carrier fluid so that the drop of reaction mixture is moved relative to the capillary injection tube;
e) repeating steps b) and c) to create an ordered succession of drops of reaction mixture in the carrier fluid to form a plurality of reactors;
f) measuring at least one representative parameter of each reactor;
g) recirculating at least one reactor to measure said at least one representative parameter over time.

3 more elements

Dependencies are inferred from the claim text.

Method For Monitoring A Reaction And To Reaction System For Implementing Same

Life of this patent

Method For Monitoring A Reaction And To Reaction System For Implementing Same

Life of this patent

Abstract

Claims23