Microfluidic electrocage device and cell medium for trapping and rotating cells for live-cell computed tomography (ct)

A method of fabricating a microfluidic device useable to perform live cell computed tomography imaging, the method comprising:

• (a) cleaning a first wafer and a second wafer;
• (b) depositing at least one metal layer on each of the first wafer and the second wafer;
• (c) depositing a positive photoresist layer over the deposited at least one metal layer of each of the first wafer and the second wafer;
• (d) impinging UV light on portions of the positive photoresist layer of each of the first wafer and the second wafer to pattern the positive photoresist layer and expose regions of the at least one metal layer on each of the first wafer and the second wafer;
• (e) wet etching the exposed regions of the least one metal layer on each of the first wafer and the second wafer to pattern the at least one metal layer;
• (f) removing the positive photoresist layer of each of the first wafer and the second wafer
• (g) defining at least one aperture through the first wafer, whereby after such at least one aperture is defined, a microfluidic device cover portion is obtained;
• (h) coating the second wafer with first negative photoresist to cover the patterned at least one metal layer;
• (i) impinging UV light on portions of the first negative photoresist coating the second wafer to pattern the first negative photoresist layer, thereby defining recesses in the first negative photoresist and exposing regions of the at least one metal layer on the second wafer, to form a microfluidic device base portion;
• and
• (j) bonding the microfluidic device cover portion and the microfluidic device base portion using a UV curable bonding agent.