Microfabricated 3D Cell Culture System
AssigneeThe Trustees of the University of Pennsylvania
InventorsChristopher S. Chen, Duc-Huy T. Nguyen, Sarah C. Stapleton, Michael T. Yang
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Abstract
Device for 3D cell culture using an extracellular matrix including a substrate having at least one interior chamber, at least one opening providing access to the interior chamber for introduction of an extracellular matrix, and at least one channel disposed through at least a portion of the extra cellular matrix.
Claims29
A device for 3D cell culture using at least one extracellular matrix, comprising:
- a) a substrate having at least one interior chamber and at least one opening providing access to the at least one chamber for introduction of the at least one extracellular matrix into the chamber;
- and
- b) at least one channel, disposed through at least a portion of the extracellular matrix for introduction of a fluid therethrough.
A method of fabricating a device for 3D cell culture, comprising:
- a) providing a top master mold for fabricating a top layer, the mold having raised portions defining at least one opening for introduction of extracellular matrix and at least one reservoir for introduction of media;
- b) casting a top layer from the top master mold;
- c) providing a bottom master mold for fabricating a bottom layer, the mold having raised portions defining an interior chamber and reservoirs for introduction of media, the reservoirs being connected to the interior chamber.
- d) casting a bottom layer from the bottom master mold;
- and
- e) treating the top layer and the bottom layer whereby the layers are adhered together.
The method of 16, wherein introduction of an angiogenic factor thereby creates a gradient extending across the extracellular matrix.
Dependencies are inferred from the claim text.