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US 2015/0086995 A1Published application
Method for detection of microorganism and kit for detection of microorganism
AssigneeMorinaga Milk Industry Co., Ltd.
InventorsShinichi Yoshida, Takashi Soejima
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Abstract
Live cells of a microorganism in a test sample are detected by the following steps: a) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison, b) the step of extracting DNA from the test sample, and amplifying a target region of the extracted DNA by PCR, and c) the step of analyzing an amplification product.
Claims20
A method for detecting live cells by distinguishing the live cells from dead cells and also from injured cells of a microorganism in a test sample, which comprises the following steps:
- a) treating the test sample with ethidium monoazide and irradiating the test sample with visible light;
- b) extracting DNA from the test sample and amplifying a target region of the extracted DNA by PCR;
- c) analyzing a resulting PCR amplification product;
- and
- d) distinguishing the live cells from dead cells and also from injured cells.
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Dependencies are inferred from the claim text.