Field-switch sequencing
AssigneePacific Biosciences of California, Inc.
InventorsJohn G. K. Williams, Jon P. Anderson
Life of this patent
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Abstract
The present invention provides novel compositions, methods and apparatus for DNA sequencing that can be performed, e.g., in a two-electrode chamber. The present invention also provides a method for sequencing a nucleic acid comprising immobilizing a plurality of complexes comprising a target nucleic acid, a primer nucleic acid, and a polymerase onto a surface, contacting the surface with a plurality of charged particles comprising a nucleotide phosphate by applying an electric field, reversing the electric field to transport unbound charged particles away from the surface, and detecting the incorporation of a nucleotide phosphate into a single molecule of the primer nucleic acid.
Claims18
A polymerase-nucleic acid complex, the polymerase-nucleic acid complex comprising:
- a target nucleic acid, a polymerase, and an attachment complex, wherein the attachment complex comprises a topological tether attached to the polymerase through at least two anchors;
- and
- wherein the attachment complex entraps the target nucleic acid.
A polymerase-nucleic acid complex, the polymerase-nucleic acid complex comprising:
- a target nucleic acid, a polymerase, and an attachment complex, wherein the attachment complex comprises a topological tether attached to the polymerase through at least two anchors;
- and
- wherein said polymerase-nucleic acid complex shows a higher processivity index than a polymerase-nucleic acid complex without said, attachment complex.
Dependencies are inferred from the claim text.