Methods and nucleic acids for analyses of cellular proliferative disorders

A method for detecting and/or classifying a colorectal carcinoma or pre-cancerous colorectal cell proliferative disorder in a human subject, comprising:

• contacting genomic DNA from a biological sample obtained from a human subject, having a colorectal carcinoma or pre-cancerous colorectal cell proliferative disorder, with at least one agent that provides for determination of a CpG methylation status of the TMEFF2 gene;
• determining, based on the contacting, a CpG methylation status of the TMEFF2 gene;
• and
• detecting and/or classifying a colorectal carcinoma or precancerous colorectal cell proliferative disorder in the subject based on increased CpG methylation of the TMEFF2 gene, relative to that of a control sample or standard value.