Methods and materials for the detection of dengue virus infection
AssigneeInBios International, Inc.
InventorsSyamal RAYCHAUDHURI, Raymond L. HOUGHTON, Stanislaw MORKOWSKI, Yvonne STEVENS, Mohua BOSE, James William NEEDHAM, Hongjing CHEN
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Abstract
The present invention provides monoclonal antibodies that are specific for the Dengue non-structural glycoprotein NS1 in monomeric and/or oligomeric (primarily dimeric) form, together with methods, including ELISA and lateral flow assays, that employ the disclosed antibodies for the early detection of Dengue virus infection. Diagnostic kits for the detection of Dengue infection are also provided, such kits including the disclosed monoclonal and/or polyclonal antibodies.
Claims21
A monoclonal antibody, or antigen binding fragment thereof, that is specific for a dengue virus NS1 polypeptide, wherein the monoclonal antibody comprises a heavy chain variable region comprising a sequence selected from the group consisting of:
- 4, 6 and 8, and a light chain variable region comprising a sequence selected from the group consisting of:
- SEQ ID NO:
- 5 and 7.
An isolated polynucleotide comprising a nucleic acid that encodes an amino acid sequence selected from the group consisting of:
- SEQ ID NO:
- 4-8.
A method for detecting dengue virus infection in a biological sample, comprising:
- (a) contacting the biological sample with a polyclonal antibody specific for a dengue NS1 polypeptide to form a polyclonal antibody-NS1 polypeptide conjugate;
- (b) contacting the polyclonal antibody-NS1 polypeptide conjugate with a monoclonal antibody of claim 1 to form a polyclonal antibody-NS1 polypeptide-monoclonal antibody conjugate;
- and
- (c) detecting the presence of the polyclonal antibody-NS1 polypeptide-monoclonal antibody conjugate, thereby detecting dengue virus infection in the biological sample. 6. The method of claim 5, wherein at least one of the monoclonal antibody and the polyclonal antibody is labeled with a detection reagent.
The method of claim 12, wherein at least one of the monoclonal antibody and the polyclonal antibody is labeled with a detection reagent.
A method for detecting dengue virus infection in a biological sample, comprising:
- (a) contacting the biological sample with a monoclonal antibody that binds to a nonhexameric form of NS1 to form a monoclonal antibody-NS1 polypeptide complex;
- and
- (b) contacting the monoclonal antibody-NS1 polypeptide complex with a detection reagent that binds to the complex, thereby detecting dengue virus infection in the biological sample.
Dependencies are inferred from the claim text.