Dual Variable Domain Immunoglobulin and Uses Thereof

A method for treating a human subject for a disease or a disorder by administering to the subject a binding protein comprising four polypeptide chains wherein:

• each of two polypeptide chains comprises VD1-(X1)n-VD2-C-(X2)n, wherein VD1 is a first heavy chain variable domain, VD2 is a second heavy chain variable domain, C is a constant domain, X1 is a linker with the proviso that it is not a constant domain, X2 is an Fc region, and n is 0 or 1;
• and
• each of two polypeptide chains comprises VD1-(X1)n-VD2-C, wherein VD1 is a first light chain variable domain, VD2 is a second light chain variable domain, C is a constant domain, and X1 is a linker with the proviso that it is not a constant domain, and n is 0 or 1;
• wherein the four polypeptide chains of the binding protein form four functional target binding sites;
• and
• wherein the binding protein binds a target or targets that are detrimental to the human subject suffering from the disease or disorder such that the activity of the target or targets in the human subject is inhibited such that treatment is achieved.