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US 2015/0079634 A1Published application
Reduction of formation of amidated amino acids in cell lines for protein expression
AssigneeLek Pharmaceuticals D.D.
InventorsMihaela Skulj, Dominik Gaser
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Abstract
The present invention is related to a method to reduce peptide amidation activity in a given cell line, cell lines with reduced peptide amidation activity, and uses thereof.
Claims25
1-15. (canceled)
A cell for protein expression, wherein the cell has reduced peptide amidation activity.
A method of reducing peptide amidation activity in a cell, wherein said method comprises at least one step selected from the group consisting of
- a) inhibition or reduction of gene expression of a gene encoding for an enzyme catalysing peptide α-amidation;
- b) expression of a dysfunctional, or inactive enzyme catalysing peptide α-amidation, or an enzyme catalysing peptide amidation with reduced activity;
- and/or
- c) inhibition or reduction of the activity of an enzyme catalysing peptide α-amidation.
1 more element
Dependencies are inferred from the claim text.