Genetically Modified Organisms for Increased Microbial Production of 3-Hydroxypropionic Acid Involving an Oxaloacetate alpha-decarboxylase
AssigneeOPX Biotechnologies, Inc.
InventorsMichael D. Lynch, Christopher P. Mercogliano
Life of this patent
Estimating the term…
Abstract
Microorganism compositions are described that comprise combinations of genetic modifications that include a genetic modification to increase oxaloacetate alpha-decarboxylase enzymatic activity. By such genetic modification a 3-hydroxypropionic acid (“3-HP”) production pathway is provided or improved. In various embodiments, comprising other genetic modifications, including selected gene disruptions, 3-HP production is greater than in a control microorganism lacking such combinations of genetic modifications
Claims33
1-43. (canceled)
A method for producing an acrylic acid-based consumer product comprising
- i) combining a carbon source and a microorganism to produce 3-hydroxypropionic acid, wherein said microorganism comprises
- a) a heterologous nucleic acid sequence encoding an enzyme selected from the group consisting of:
- a native or mutated form of a mmsB protein, and a native or mutated form of a ydfG protein;
- b) a genetic modification to decrease or eliminate an enzymatic activity selected from the group consisting of:
- lactate dehydrogenase, phosphate acetyltransferase, and pyruvate-formate lyase;
- c) a heterologous nucleic acid sequence encoding an acetyl-CoA carboxylase;
- and
- d) a heterologous nucleic acid sequence encoding a cyanase,
- ii) converting said 3-hydroxypropionic acid to acrylic acid;
- and
- iii) processing said acrylic acid into a consumer product.
A method for producing an acrylic acid-based consumer product comprising
- i) combining a carbon source and a microorganism to produce 3-hydroxypropionic acid, wherein said microorganism comprises
- a) a heterologous nucleic acid sequence encoding a 3-hydroxypropionate dehydrogenase;
- b) a heterologous nucleic acid sequence encoding a cyanase or a carbonic anhydrase;
- and
- c) a heterologous nucleic acid sequence encoding an NADPH-dependent transhydrogenase or an acetyl-CoA carboxylase;
- ii) converting said 3-hydroxypropionic acid to acrylic acid;
- and
- iii) processing said acrylic acid into a consumer product.
A method for producing an acrylic acid-based consumer product comprising
- i) combining a carbon source and a microorganism to produce 3-hydroxypropionic acid, wherein said microorganism comprises
- a) a heterologous nucleic acid sequence encoding a 3-hydroxypropionate dehydrogenase;
- and
- b) a heterologous nucleic acid sequence encoding a cyanase or a carbonic anhydrase,
- ii) converting said 3-hydroxypropionic acid to acrylic acid;
- and
- iii) processing said acrylic acid into a consumer product.
Dependencies are inferred from the claim text.