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US 2015/0071877 A1Published application
Bioactive compositions and methods for their preparation and use
AssigneeBioregenerative Sciences
InventorsGreg Maguire, Peter Friedman
Life of this patent
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Abstract
A method for preparing a bioactive composition containing conditioned cell culture medium is disclosed. The method comprises culturing cells of two or more eukaryotic cell line to form conditioned culture media, separating the cultured cells from the conditioned culture media, and combining conditioned culture media to form a bioactive composition. Novel bioactive compositions, formulations and their use in treating of a variety of diseases and health conditions are also disclosed.
Claims37
A method for making a bioactive composition comprising:
- culturing cells of a first cell line and a second cell line in a first and a second culture medium, wherein said cells secrete extracellular products into the respective culture medium so that a first conditioned culture medium and a second conditioned culture medium are respectively formed;
- optionally culturing cells of a third cell line in a third culture medium, wherein said cells secrete extracellular products into the culture medium so that a third conditioned culture medium is formed;
- separating said first, second, and optionally third conditioned culture media from respective said cultured cells;
- and
- combining said first and said second conditioned culture media, and optionally said third conditioned culture medium, to form a bioactive composition,
- wherein said first, second, and optionally third cell lines are different from one another and are each selected from the group consisting of an adipose-derived stem cell (ADSC) line, a non-adipose mesenchymal stem cell line, a fibroblast cell line, a hair dermal papilla (HDP) cell line, and a limbal stem cell line.
3 more elements
A bioactive formulation comprising a composition prepared by
- culturing cells of a first cell line and a second cell line in a first and a second culture medium, wherein said cells secrete extracellular products into said culture medium so that a first conditioned culture medium and a second conditioned culture medium are formed;
- optionally culturing cells of a third cell line in a third culture medium, wherein said cells secrete extracellular products into the culture medium so that a third conditioned culture medium is formed;
- separating said first, second, and optionally third conditioned culture media from respective said cultured cells;
- and
- combining said first and said second conditioned culture media, and optionally said third conditioned culture medium to form the composition,
- wherein said first, second, and optionally third cell lines are different from one another and are each selected from the group consisting of an adipose derived stem cell (ADSC) line, a non-adipose mesenchymal stem cell line, a fibroblast cell line, a hair dermal papilla (HDP) cell line, and a limbal stem cell line.
3 more elements
Dependencies are inferred from the claim text.