Citrus plants resistant to citrus huanglongbing (ex greening) caused by candidatus liberibacter asiaticus (las) and bacterial canker caused by (xanthomonas axonopodis pv. citri) (xac)
AssigneeSouthern Gardens Citrus
InventorRicke Kress
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Abstract
Disclosed herein are viral vectors based on modifications of the Citrus Tristeza virus useful for transfecting citrus trees for beneficial purposes. Included in the disclosure are viral vectors including one or more gene cassettes that encode spinach defensin peptide(s). The gene cassettes are positioned at desirable locations on the viral genome so as to enable expression while preserving functionality of the virus. Also disclosed are methods of transfecting plants and plants transfected with viral vector embodiments.
Claims31
A CTV viral vector engineered to comprise at least one gene cassette comprising a polynucleotide encoding at least one spinach defensin peptide, the CTV viral vector engineered such that the gene cassette is positioned at CTV genome regions p13-p20, p20-p2, p23-3′NTR, or positioned in place of p13, or positioned after p13 or p23 or between the minor coat protein and the coat protein.
A CTV viral vector engineered to comprise a gene cassette comprising a first polynucleotide sequence encoding a first spinach defensin peptide, a protease with cleavage sites engineered on each end, and a second polynucleotide sequence encoding a second spinach defensin peptide, wherein the spinach defensin peptides are the same or different.
A CTV viral vector engineered to comprise a gene cassette comprising a polynucleotide sequence encoding at least one spinach defensin peptide and a protease with cleavage sites engineered on each end fused in the same reading frame with a viral protein.
A CTV viral vector engineered to comprise a first gene cassette comprising a first polynucleotide sequence encoding a first spinach peptide and a first controller element upstream of said first polynucleotide sequence;
- and a second gene cassette comprising a second polynucleotide sequence encoding a second spinach peptide and a second control element upstream of said second polynucleotide sequence.
Dependencies are inferred from the claim text.