Method for measuring radioactivity
AssigneesLLari Maasilta, Marja Tiirola
InventorMarja Tiirola
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Abstract
The present invention provides a method for determining radioactivity in an ion-sensitive field effect transistor array, the method comprising the steps of incubating the array with electron-sensitive silver-halide material and then incubating the array with a developer solution reducing the silver halides that have been exposed to radioactivity to elemental silver and simultaneously measuring pH at each separate reaction chambers, wherein decrease of the pH indicates the presence of radioactivity in a reaction chamber.
Claims12
Method for determining radioactivity in an ion-sensitive field effect transistor array comprising the steps of a) attaching molecules of a sample to reaction chambers in said array;
- b) if said sample does not contain radioactivity, contacting said array with a radioactive molecule or radioactive molecules with specific binding properties;
- c) incubating the array with electron-sensitive silver-halide material;
- and d) incubating the array with a developer solution reducing the silver halides that have been exposed to radioactivity to elemental silver and simultaneously measuring pH at each separate reaction chamber, wherein decrease of the pH indicates the presence of radioactivity in a reaction chamber.
Method for determining chemiluminescence in an ion-sensitive field effect transistor array comprising the steps of a) attaching molecules of a sample to reaction chambers in said array;
- b) contacting said array with a chemiluminescence causing enzymatic molecule or enzymatic molecules that are conjugated to molecules with specific binding properties;
- c) incubating the array with electron-sensitive silver-halide material and chemiluminescence substrate;
- and d) incubating the array with a developer solution reducing the silver halides that have been exposed to chemiluminescence light to elemental silver and simultaneously measuring pH at each separate reaction chamber, wherein decrease of the pH indicates the presence of chemiluminescence in a reaction chamber.
Dependencies are inferred from the claim text.