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US 2015/0064743 A1Published application
Methods and Materials for Biosynthesis of Mogroside Compounds
AssigneeEvolva SA
InventorsYaoquan Liu, Jorgen Hansen, Jens Houghton-Larsen, Muthuswamy Panchapagesa Murali, Sathish Kumar, Nina Nicoline Rasmussen
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Abstract
The invention relates to methods for producing mogrosides with the aid of enzymes. In particular the invention proposes various biosynthetic pathways useful for mogroside production and enzymes useful for mogroside production are provided. Furthermore, the invention provides recombinant hosts useful in performing the methods of the invention.
Claims26
A method of producing a mogroside compound, said method comprising one or more of the following steps:
- Step IIb. Contacting dioxido-squalene with an enzyme capable of catalyzing conversion of Dioxido-squalene->24,25 epoxy cucurbitadienol;
- Step IVa. Contacting 11-hydroxy-cucurbitadienol with an enzyme or mixture of enzymes capable of catalysing conversion of 11-hydroxy-cucurbitadienol->mogrol;
- Step IVb. Contacting 11-hydroxy-24,25 epoxy cucurbitadienol with an enzyme capable of catalysing conversion of 11-hydroxy-24,25 epoxy cucurbitadienol->mogrol;
- Step V. Contacting mogrol and/or glucosylated mogrol with an enzyme or mixture of enzymes capable of catalysing glucosylation of mogrol and/or glucosylated mogrol to form mogroside, wherein said enzyme or mixture of enzyme comprises a UGT selected from the group consisting of UGT1576, UGT98 and UGT SK98.
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A recombinant host cell comprising one or more of the following heterologous nucleic acids:
- IIb. Heterologous nucleic acid encoding an enzyme capable of catalyzing conversion of Dioxido-squalene->24,25 epoxy cucurbitadienol;
- IVa Heterologous nucleic acid(s) encoding an enzyme or mixture of enzymes capable of catalysing conversion of 11-hydroxy-cucurbitadienol->mogrol;
- IVb Heterologous nucleic acid encoding an enzyme capable of catalysing conversion of 11-hydroxy-24,25 epoxy cucurbitadienol->mogrol;
- V. Heterologous nucleic acid(s) encoding an enzyme or mixture of enzymes capable of catalysing glucosylation of mogrol and/or glucosylated mogrol to form mogroside, wherein said enzyme or mixture of enzyme comprises a UGT selected from the group consisting of UGT1576, UGT98 and UGT SK98.
1 more element
Dependencies are inferred from the claim text.