Recombinant papaya mosaic virus coat proteins and uses thereof in influenza vaccines
AssigneeFolia Biotech Inc.
InventorsDenis Leclerc, Nathalie Majeau
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Abstract
Recombinant papaya mosaic virus (PapMV) coat proteins comprising one or more antigenic peptides derived from an influenza virus antigen, such as from the M2e peptide, fused at a position within a predicted random coil within 13 amino acids of the N-terminus of the coat protein, uses thereof to prepare virus-like particles (VLPs), and uses of the VLPs in influenza vaccines.
Claims31
A fusion protein comprising a peptide antigen derived from influenza M2e peptide fused to a papaya mosaic virus (PapMV) coat protein after an amino acid that corresponds to amino acid 6, 7, 8, 9, 10, 11 or 12 of SEQ ID NO:1, wherein the fusion protein is capable of self-assembly to form a virus-like particle (VLP), and wherein the peptide antigen is 20 amino acids or less in length and comprises the general sequence:
- V-X1-T-X2-X3-X4-X5 [SEQ ID NO:96], wherein X1 is E or D;
- X2 is P or L;
- X3 is T or I;
- X4 is R or K, and X5 is N, S or K.
The fusion protein according to any one of claims 1 to 8, wherein the peptide antigen comprises a sequence as set forth in any one of SEQ ID NOs:14-22 and 96-104.
Dependencies are inferred from the claim text.