Method of Bonding Gold Nanoparticles with Diethylenetriamine Pentaacetic Acid

A method of bonding gold nanoparticles (GNPs) with diethylenetriamine pentaacetic acid (DTPA), comprising steps of:

• (a) obtaining 1 mL±10% of 0.1 M of gold chloride (HAuCl4) to be dissolved into 500 mL±10% of ultrapure water;
• and adding 10 mL±10% of sodium borohydride (NaBH4) with stirring to obtain a solution of GNPs;
• (b) obtaining 1 mL±10% of bovine serum albumin (BSA) with secondary water;
• adding 2 mL±10% of said solution of GNPs to adhere BSA on surface of said GNPs;
• processing purification;
• and obtaining liquid phase of a phosphate buffer;
• and
• (c) obtaining 50 mg±10% of BSA in 0.1 M of a carbonate-bicarbonate buffer to be reacted with 1 mg±10% of a DTPA in a volume of 1 mL;
• processing a first purification;
• adding 2 mL of said solution of GNPs to be reacted;
• and obtaining liquid phase of a phosphate buffer.