Novel plant-derived cis-regulatory elements for the development of pathogen-responsive chimeric promotors
AssigneeKws Saat AG
InventorsDietmar Stahl, Fridtjof Weltmeoer, Reinhard Hehl, Jeannette Koschmann, Julia Niemeyer
Life of this patent
Estimating the term…
Abstract
The invention relates to an isolated cis-regulatory element imparting pathogen inducibility or elicitor inducibility, which comprises a nucleic acid molecule, the nucleotide sequence of which corresponds to one of the core sequence motifs comprising a) vaaagtm, b) aaacca, c) scaaam, d) acrcg, e) sktgkact, f) mrtsack, g) ccaccaa, h) tcgtctcttc (SEQ ID NO: 35), i) wwkgwc or a core sequence motif complementary to a) to i).
Claims27
1-5. (canceled)
A chimeric promoter which is suitable for effecting an expression of an operatively linked nucleic acid molecule in a plant cell and which comprises a minimal promoter and, upstream from the minimal promoter, at least one cis-regulatory element wherein said cis-regulatory element imparts a pathogen inducibility or an elicitor inducibility and comprises a-nucleotide sequence selected from the group consisting of
- a)
- vaaagtm,
- (SEQ ID NO:
- 138)
- b)
- aaacca,
- (SEQ ID NO:
- 139)
- c)
- scaaam,
- (SEQ ID NO:
- 140)
- d)
- acrcg,
- (SEQ ID NO:
- 141)
- e)
- sktgkact,
- (SEQ ID NO:
- 142)
- f)
- mrtsack,
- (SEQ ID NO:
- 143)
- g)
- ccaccaa,
- (SEQ ID NO:
- 144)
- h)
- tcgtctcttc,
- (SEQ ID NO:
- 35)
- i)
- wwkgwc,
- (SEQ ID NO:
- 145)
- and
- j) a nucleotide sequence complementary to any one of the sequences a) to i).
Dependencies are inferred from the claim text.