Methods and compositions for the delivery of nucleic acids to seeds
AssigneeMorflora Israel Ltd.
InventorsMIRI MIRIAM LAPIDOT, IRIS SMIRRA, HERVE HUET, DOTAN PELEG, OMRI LEMPEL
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Abstract
The present invention relates to methods of seed treatment and introduction of nucleic acid particles into intact seeds. In particular, the methods are non-priming seed treatment protocols capable of delivering naked DNA plasmids into seeds, without the use of microorganism or any additional means, and which are not plant species limited.
Claims20
A method for introducing nucleic acid into at least one cell of a plant seed embryo comprising contacting a plant seed for less than 60 minutes with a non-priming medium comprising a naked nucleic acid construct or a virus-based DNA construct comprising heterologous DNA, which results in a seed water content increase of less than 20% w/w, thereby obtaining the seed uploaded with the nucleic acid.
Dependencies are inferred from the claim text.