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US 2015/0040266 A1Published application
Use of non-agrobacterium bacterial species for plant transformation
AssigneeMonsanto Technology LLC
InventorsXudong Ye, Junjiang Shen, Edward Williams
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Abstract
The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.
Claims27
A method for transforming a plant cell, comprising:
- (a) contacting at least a first plant cell with a Rhizobiales bacterium other than Agrobacterium sp. comprising:
- (i) a first nucleic acid comprising a vir gene region of a Ti plasmid wherein the vir gene region acts to introduce a nucleic acid of interest into the plant cell in a VirD2-dependent manner;
- and (ii) a second nucleic acid comprising one or more T-DNA border sequence(s) operably linked to a nucleic acid of interest;
- and
- (b) selecting at least a first plant cell transformed with the nucleic acid of interest, wherein the plant cell is a soybean, canola, or cotton plant cell.
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Dependencies are inferred from the claim text.