GRF3 Mutants, Methods and Plants
AssigneesUniversidad Nacional De Rosario, Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet)
InventorsJavier Palatnik, Ramiro Rodriguez, Martin Mecchia, Juan Manuel Debernandi
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Abstract
The present disclosure provides a novel modified gene, rGRF3, or an orthologue thereof, which is shown to be decoupled from control by miR396, particularly in the presence of over-expression of at least one GIF gene, such as GIF1, AtGIF 2, AtGIF 3, Os11g40100, Os12g31350, Os03g52320 or combinations thereof. When present in a plant, the rGRF3 results in a phenotype of increased productivity (e.g. increased yield, increased biomass, increased stress resistance, increased seed production, increased seed yield, increased root growth, increased root elongation speed, delayed leaf senescence or increased drought tolerance and combinations thereof).
Claims39
An isolated nucleic acid encoding a modified growth regulatory factor (GRF)-3 or an orthologue thereof which nucleic acid is decoupled from control by miR396.
An isolated nucleic acid which comprises (or consists of) a modified GRF-3 gene which is decoupled from control by miR396.
An isolated nucleic acid according to any one of the preceding claims wherein the nucleic acid encodes a protein having the FFD (for example a FFD(D/E)WP) motif.
An isolated nucleic acid according to any one of the preceding claims wherein the growth regulatory factor (GRF) is a GRF selected from the group consisting of:
- Arabidopsis thaliana GRF 3 or 4;
- Oryza sativa GRF 1, 2, 3. 4 or 5;
- Zea mays GRF 1, 3, 5, 6, 7, 9, 11 or 14;
- Glycine max GRF;
- Medicago truncatula GRF;
- Populus trichocarpa GRF, Carica papaya GRF and Prunus persica GRF which have been decoupled from control by miR396.
An isolated nucleic acid according to any one of the preceding claims comprising i) a nucleotide sequence shown as SEQ ID No. 2 (AtGRF3);
- ii) or a nucleotide sequence which is at least 45%, preferably at least 50%, preferably at least 60%, preferably at least 65%, identical to SEQ ID No. 2;
- or iii) a nucleotide sequence which hybridises under stringent conditions with a nucleotide sequence of either i) or ii) wherein the nucleotide sequence comprises a modification in the miR396 target site to decouple the nucleic acid from control by miR396.
An isolated nucleic acid according to any one of the preceding claims wherein the GRF-3 or orthologue thereof decoupled from miR396 regulation exhibits enhanced performance in combination with the over-expression of at least one GIF gene, such as GIF1, AtGIF 2, AtGIF 3, Os11g40100, Os12g31350, Os03g52320 or combinations thereof.
An isolated nucleic acid according to any one of the preceding claims wherein the modified GRF3 or orthologue thereof comprising at least one or all of the following base changes in the miR396 target site an A->U, a G->A, a U->A, a U->G, a G->C, a A->T, a G->A, a T->A, a G->A, a A->G modification, all of which retain the native amino acid sequence, but which substantially destabilize the interaction of miR396 with said rGRF3.
An isolated nucleic acid according to any one of the preceding claims wherein the modified GRF 3 or orthologue thereof comprises a modified miR396 target site having the following sequence:
- CGTTCxAGAAAxCCxGTxGAx (SEQ ID No. 86), wherein x designates bases that have been modified (e.g. compared with the wild-type sequence).
An isolated nucleic acid according to any one of the preceding claims wherein the modified GRF 3 or orthologue thereof comprises a modified miR396 target site having the following sequence:
- CGTTCtAGAAAaCCaGTaGAg (SEQ ID No. 38), wherein the lower case letters designates modified bases.
A nucleic acid, a construct, a vector, a plant, plant cell or plant tissue, a method or a use as described generally herein with reference to the Examples and Figures.
Dependencies are inferred from the claim text.