Methods and compositions for emergency post-infection treatment of anthrax
AssigneePharma Green LLC
InventorsVyacheslav Andrianov, Maxim Golovkin
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Abstract
Compositions effective for treating or preventing an anthrax infection in a subject in need thereof and recombinant proteins included in the compositions are provided. Methods for producing recombinant proteins in plants are described. Transgenic plants engineered to produce recombinant proteins as well as genetic constructs comprising nucleic acids encoding recombinant proteins thereof are also described. Methods of protecting subjects against anthrax infection with plant-derived compositions are provided.
Claims50
A recombinant protein comprising a first protein fused to a second protein, wherein the first protein is a toxin binding ligand and the second protein is a carrier-protein selected from the group consisting of:
- Fc-IgA, Fc-IgG, Fc-IgM, oleosin, and VP2 coat protein.
A genetic construct comprising a first polynucleotide and a second polynucleotide, wherein the first polynucleotide encodes a toxin binding ligand, and the second polynucleotide encodes a carrier-protein selected from the group consisting of:
- Fc-IgA, Fc-IgG, Fc-IgM, oleosin, and VP2 coat protein.
A genetic construct comprising a sequence with at least 90% identity to a reference sequence selected from the group consisting of:
- SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEQ ID NO:
- 23, SEQ ID NO:
- 37, and SEQ ID NO:
- 38.
A transgenic plant comprising a genetic construct that includes a first polynucleotide and a second polynucleotide, wherein the first polynucleotide encodes a toxin binding ligand, the second polynucleotide encodes a carrier-protein and the carrier-protein is selected from the group consisting of:
- Fc-IgA, Fc-IgG, Fc-IgM, oleosin, and VP2 coat protein.
A method for producing a recombinant protein in a plant comprising:
- contacting a plant with a genetic construct that comprises a nucleic acid encoding the recombinant protein, wherein the recombinant protein includes a toxin binding ligand fused to a carrier-protein, and the carrier-protein is selected from the group consisting of:
- Fc-IgA, Fc-IgG, Fc-IgM, oleosin, and VP2 coat protein;
- obtaining a plant including the genetic construct and expressing the recombinant protein.
A method of protecting a subject against anthrax infection comprising:
- providing a composition that includes a recombinant protein, wherein the recombinant protein includes a toxin binding ligand fused to a carrier-protein, and the carrier protein is a protein selected from the group consisting of:
- Fc-IgA, Fc-IgG, Fc-IgM, oleosin, and VP2 coat protein, wherein the composition is effective in preventing or reducing at least one symptom of an anthrax infection in a subject;
- and
- administering the composition to the subject in need thereof.
Dependencies are inferred from the claim text.