Expression cassettes for regulation of expression in monocotyledonous plants

A method for providing a transgenic expression cassette for heterologous expression in a monocotyledonous plant, said method comprising:

• a) isolating a transcription regulating nucleotide sequence from a monocotyledonous plant, wherein the transcription regulating nucleotide sequence shares at least 90% sequence identity to the nucleotide sequence of SEQ ID NO:
• 2, 3, 6, 7, 8, 11, 12, 13, 14, 15, 16, 19, 20, 21, 22, 23, 24, 27, 28, 29, 56, 57, 58, 61, 62, 63, 66, 67, 68, 71, 72, or 73;
• and
• b) functionally linking said transcription regulating nucleotide sequence to another nucleotide sequence of interest, which is heterologous in relation to said transcription regulating nucleotide sequence.

A method for identifying and/or isolating a sequence with transcription regulating activity, said method comprising:

• a) obtaining polynucleotide sequences sharing at least 90% sequence identity with the nucleotide sequence of SEQ ID NO:
• 2, 3, 6, 7, 8, 11, 12, 13, 14, 15, 16, 19, 20, 21, 22, 23, 24, 27, 28, 29, 56, 57, 58, 61, 62, 63, 66, 67, 68, 71, 72, or 73;
• b) preparing expression cassettes comprising any of the polynucleotide sequences of a) operably linked to a reporter gene or marker and optionally a terminator sequence;
• c) testing the expression cassettes for expression activity of the polynucleotide sequence comprised therein;
• and
• d) identifying and/or isolating a polynucleotide sequence having transcription regulating activity.