Plants having improved growth properties
AssigneeSweTree Technologies AB
InventorsMaria Eriksson, Naoki Takata, Mikael Johansson
Life of this patent
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Abstract
The invention relates to a method for producing a genetically modified plant with improved growth properties as compared to a corresponding non-genetically modified wild type plant, said method comprising reducing or deleting the amount or activity of an EBI1 or EBI2 polypeptide in a plant cell, a plant or a part thereof.
Claims16
A method for producing a genetically modified plant with improved growth properties as compared to a corresponding non-genetically modified wild type plant, said method comprising:
- a) reducing or deleting the amount or activity of an EBI1 or EBI2 polypeptide in a plant cell, a plant or a part thereof to produce a modified plant cell, plant or a part thereof;
- b) generating, from the modified plant cell, plant or part thereof in step a) a genetically modified plant;
- c) identifying a genetically modified plant from step b) with improved growth properties as compared to a corresponding non-genetically modified wild type plant;
- and
- d) growing said genetically modified plant under conditions which permit the development of the genetically modified plant.
A genetically modified plant having improved growth properties as compared to a corresponding non-genetically modified wild type plant, wherein said plant has a reduced amount or activity of a EBI1 or EBI2 polypeptide, and wherein the genome of said plant comprises a genetic modification selected from any one of:
- i) a non-silent mutation in an endogenous gene comprising a nucleic acid molecule encoding an EBI1 or EBI2 polypeptide;
- ii) a transgene inserted into said genome, said transgene comprising a nucleic acid molecule encoding a ribonucleic acid sequence, which is able to form a double-stranded ribonucleic acid molecule, whereby a fragment of at least 17 nucleotides of said double-stranded ribonucleic acid molecule has a homology of at least 50% to a nucleic acid molecule encoding an EBI1 or EBI2 polypeptide;
- iii) a mutation in an endogenous gene comprising a nucleic acid molecule encoding an EBI1 or EBI2 polypeptide, induced by introducing into at least one plant cell a nucleic acid construct able to recombine with and silence, inactivate, or reduce the activity of the endogenous gene,
- wherein said EBI1 polypeptide has an amino acid sequence having at least 80% amino acid sequence identity to a sequence selected from among SEQ ID NOS:
- 2, 4 and 5, or wherein said EBI2 polypeptide has an amino acid sequence having at least 80% amino acid sequence identity to a sequence selected from among SEQ ID NOS:
- 7, 9 and 10;
- and
- wherein said genetically modified plant has improved growth properties as compared to a corresponding non-genetically modified wild type plant.
Dependencies are inferred from the claim text.