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US 2015/0033370 A1Published application
Model and method for a transgenic bovidae expressing cardiac fibrosis and associated pathology
AssigneeUtah State University
InventorsIrina Polejaeva, Zhongde Wang, Shengwei Hu, Ravi Ranjan, Aaron James Thomas, Christopher Joseph Davies, Kenneth L. White
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Abstract
Herein provided are a model and method for a transgenic a bovidae having an TGF-β1 gene inserted into the bovidae genome and capable of expressing higher than normal levels of TGF-β1 in cardiac muscle.
Claims20
A transgenic bovidae model expressing cardiac fibrosis, comprising:
- a transgenic bovidae having an hTGF-β1 gene inserted randomly or into an appropriate locus of the bovidae genome, wherein the bovidae is capable of expressing higher than normal levels of active TGF-β1 in cardiac muscle.
A method for at least one of a bovidae embryo, blastocyst, fetus or animal expressing cardiac fibrosis TGF-β1, the method comprising:
- producing a cytoplast by enucleation of genetic material from an oocyte;
- inserting a transfected fibroblast donor cell comprising a TGF-β1 under control of α-MHC promoter inserted AAVS1 locus into the cytopoast;
- fusing at least one karyoplast with one cytoplast resulting in a reconstructed embryo;
- activating the reconstructed embryo to induce embryonic development;
- and
- transferring the reconstructed embryo into a surrogate recipient bovidae immediately after activation or after in vitro embryo culture such that the embryo develops into a genetically modified fetus.
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