Ultra-sensitive chemiluminescent substrates for enzymes and their conjugates
AssigneeMichigan Diagnostics, LLC
InventorsBrij Pal Giri, Dinesh J. Dagli, Pritam Singh
Life of this patent
Estimating the term…
Abstract
New chemiluminescent compounds, stable in aqueous buffers, for use in biological assaying include acridane-based compounds and 1,2-dioxetanes. Among the new acridane-based compounds are water-soluble acridanes, enhancer coupled acridanes, bis and tris-acridanes as well as acridane-1,2-dioxetanes. Among the new 1,2-dioxetanes are electron deficient group-containing dioxetanes and tethered bis-1,2-dioxetanes. The 1,2-dioxetanes are useful as substrates for various enzymes. The acridanes can be admixed with an oxidizing agent, an aqueous buffer and, optionally, a stabilizer to form a substrate or reagent formulation useful for assaying, inter alia, HRP.
Claims1
A chemiluminescent assay formulation, comprising:
- at least one chemiluminescent compound which is a 1,2-dioxetane coupled acridane corresponding to the formula:
- wherein R12 a is substituted 1,2-dioxetane, R13, is a linear or branched C1 to C6 alkyl or deuterated alkyl, aryl or deuterated aryl, arylalkyl, alkylaryl, heteroalkyl, alkylalkene, arylalkene, alkylnitrile, alkylalcohol and alkyacid;
- R14 and R15 may be alkyl or deuterated alkyl, methoxy or deuterated methoxy, Cl, Br or CN, and wherein at least one of R12, R13, R14, and R15 is a deuterium atom or deuterium atom containing organic group and X is oxygen, nitrogen or sulfur.
Dependencies are inferred from the claim text.